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Title: Efficient delivery of immunostimulatory DNA to mouse and human immune cells through the construction of polypod-like structured DNA.
Authors: Uno, Shota
Nishikawa, Makiya  kyouindb  KAKEN_id
Mohri, Kohta
Umeki, Yuka
Matsuzaki, Noriyuki
Takahashi, Yuki  kyouindb  KAKEN_id
Fujita, Haruyuki
Kadowaki, Norimitsu  KAKEN_id
Takakura, Yoshinobu  kyouindb  KAKEN_id
Author's alias: 西川, 元也
Keywords: CpG DNA
Toll-like receptor 9
Dendritic cell
Peripheral blood mononuclear cell
Issue Date: May-2014
Publisher: Elsevier Inc.
Journal title: Nanomedicine
Volume: 10
Issue: 4
Start page: 765
End page: 774
Abstract: Investigation of mouse macrophage-like RAW264.7 cells showed that the immunostimulatory activity of CpG DNA is increased by formation of polypod-like structured DNA (polypodna), an assembly consisting of three or more oligodeoxynucleotides. To apply CpG polypodna to immunotherapy, its activity was examined in murine dendritic DC2.4 cells, splenic macrophages, and bone marrow-derived dendritic cells (BMDCs). In all cell types, increasing the pod number increased the cellular uptake of DNA and cytokine release. No significant release of cytokines was observed in macrophages lacking Toll-like receptor 9. Similar results were obtained after intradermal injection of polypodna. The polypodna preparations produced significantly higher amounts of interferon α in human peripheral blood mononuclear cells (PBMCs) compared with single-stranded DNA. The conditioned medium of hexapodna-treated human PBMCs effectively inhibited the activity of a hepatitis C virus subgenomic replicon reporter system. These results indicate that polypodna preparations are useful as an immunostimulator.
Rights: © 2014 Elsevier Inc.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/187794
DOI(Published Version): 10.1016/j.nano.2013.11.017
PubMed ID: 24333587
Appears in Collections:Journal Articles

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