Access count of this item: 139

Files in This Item:
File Description SizeFormat 
srep05281.pdf898.53 kBAdobe PDFView/Open
Title: Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface.
Authors: Ikuta, Junya
Kamisetty, Nagendra K
Shintaku, Hirofumi  kyouindb  KAKEN_id
Kotera, Hidetoshi
Kon, Takahide
Yokokawa, Ryuji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6306-2693 (unconfirmed)
Author's alias: 生田, 純也
Kamisetty, Nagendra K
新宅, 博文
小寺, 秀俊
昆, 隆英
横川, 隆司
Keywords: Molecular biophysics
Nanofabrication and nanopatterning
Issue Date: 2014
Publisher: Nature Publishing Group
Journal title: Scientific reports
Volume: 4
Thesis number: 5281
Abstract: Intracellular cargo is transported by multiple motor proteins. Because of the force balance of motors with mixed polarities, cargo moves bidirectionally to achieve biological functions. Here, we propose a microtubule gliding assay for a tug-of-war study of kinesin and dynein. A boundary of the two motor groups is created by photolithographically patterning gold to selectively attach kinesin to the glass and dynein to the gold surface using a self-assembled monolayer. The relationship between the ratio of two antagonistic motor numbers and the velocity is derived from a force-velocity relationship for each motor to calculate the detachment force and motor backward velocity. Although the tug-of-war involves >100 motors, values are calculated for a single molecule and reflect the collective dynein and non-collective kinesin functions when they work as a team. This assay would be useful for detailed in vitro analysis of intracellular motility, e.g., mitosis, where a large number of motors with mixed polarities are involved.
Description: 「分子綱引き」をおこなうナノシステムを開発 -細胞分裂や細胞内物質輸送の仕組みを知るカギに-.京都大学プレスリリース. 2014-06-17.
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
URI: http://hdl.handle.net/2433/188352
DOI(Published Version): 10.1038/srep05281
PubMed ID: 24923426
Related Link: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2014/140613_1.htm
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.