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Title: High-throughput sequencing shows inconsistent results with a microscope-based analysis of the soil prokaryotic community
Authors: Ushio, Masayuki
Makoto, Kobayashi
Klaminder, Jonatan
Takasu, Hiroyuki
Nakano, Shin-ichi  kyouindb  KAKEN_id
Author's alias: 潮, 雅之
Keywords: Archaea
High-throughput sequencing
Soil prokaryotic community
Tundra ecosystem
Issue Date: Sep-2014
Publisher: Elsevier Ltd.
Journal title: Soil Biology and Biochemistry
Volume: 76
Start page: 53
End page: 56
Abstract: In the present study, we perform the first direct analysis on how the composition of the prokaryotic soil community differs depending on whether high-throughput sequencing or fluorescent in situ hybridization (FISH) coupled with catalyzed reporter deposition (CARD) is used. Soil samples were collected along short (<3 m) tundra vegetation gradients from Northern Sweden. Relative abundances of Acidobacteria and Bacteroidetes estimated by the high-throughput sequencing were higher than those estimated by CARD–FISH, while relative abundances of Archaea and α-Proteobacteria estimated by high-throughput sequencing were lower than those estimated by CARD–FISH. The results indicated that the high-throughput sequencing overestimates/underestimates the relative abundance of some microbial taxa if we assume that CARD–FISH can provide potentially more quantitative data. Great caution should be taken when interpreting data generated by molecular technologies (both of high-throughput sequencing and CARD–FISH), and supports by multiple approaches are necessary to make a robust conclusion.
Rights: © 2014 Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.soilbio.2014.05.010
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