ダウンロード数: 504
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.soilbio.2014.05.010.pdf | 4.9 MB | Adobe PDF | 見る/開く |
タイトル: | High-throughput sequencing shows inconsistent results with a microscope-based analysis of the soil prokaryotic community |
著者: | Ushio, Masayuki https://orcid.org/0000-0003-4831-7181 (unconfirmed) Makoto, Kobayashi Klaminder, Jonatan Takasu, Hiroyuki Nakano, Shin-ichi https://orcid.org/0000-0001-5235-8547 (unconfirmed) |
著者名の別形: | 潮, 雅之 |
キーワード: | Archaea Bacteria CARD–FISH High-throughput sequencing Soil prokaryotic community Tundra ecosystem |
発行日: | Sep-2014 |
出版者: | Elsevier Ltd. |
誌名: | Soil Biology and Biochemistry |
巻: | 76 |
開始ページ: | 53 |
終了ページ: | 56 |
抄録: | In the present study, we perform the first direct analysis on how the composition of the prokaryotic soil community differs depending on whether high-throughput sequencing or fluorescent in situ hybridization (FISH) coupled with catalyzed reporter deposition (CARD) is used. Soil samples were collected along short (<3 m) tundra vegetation gradients from Northern Sweden. Relative abundances of Acidobacteria and Bacteroidetes estimated by the high-throughput sequencing were higher than those estimated by CARD–FISH, while relative abundances of Archaea and α-Proteobacteria estimated by high-throughput sequencing were lower than those estimated by CARD–FISH. The results indicated that the high-throughput sequencing overestimates/underestimates the relative abundance of some microbial taxa if we assume that CARD–FISH can provide potentially more quantitative data. Great caution should be taken when interpreting data generated by molecular technologies (both of high-throughput sequencing and CARD–FISH), and supports by multiple approaches are necessary to make a robust conclusion. |
著作権等: | © 2014 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/188896 |
DOI(出版社版): | 10.1016/j.soilbio.2014.05.010 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。