ダウンロード数: 432
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.apradiso.2013.11.117.pdf | 387.53 kB | Adobe PDF | 見る/開く |
タイトル: | Dose-rate effect was observed in T98G glioma cells following BNCT. |
著者: | Kinashi, Yuko ![]() Okumura, Kakuji Kubota, Yoshihisa Kitajima, Erika Okayasu, Ryuichi Ono, Koji Takahashi, Sentaro ![]() |
著者名の別形: | 木梨, 友子 |
キーワード: | Dose-rate effect BNCT DNA-DSBs Glioma |
発行日: | Jun-2014 |
出版者: | Elsevier Ltd. |
誌名: | Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine |
巻: | 88 |
開始ページ: | 81 |
終了ページ: | 85 |
抄録: | [Background]It is generally said that low LET radiation produce high dose-rate effect, on the other hand, no significant dose rate effect is observed in high LET radiation. Although high LET radiations are produced in BNCT, little is known about dose-rate effect of BNCT. [Materials and methods]T98G cells, which were tumor cells, were irradiated by neutron mixed beam with BPA. As normal tissue derived cells, Chinese hamster ovary (CHO-K1) cells and DNA double strand breaks (DNA-DSBs) repair deficient cells, xrs5 cells were irradiated by the neutrons (not including BPA). To DNA-DSBs analysis, T98G cells were stained immunochemically with 53BP1 antibody. The number of DNA-DSBs was determined by counting 53BP1 foci. [Results]There was no dose-rate effect in xrs5 cells. D0 difference between 4 cGy/min and 20 cGy/min irradiation were 0.5 and 5.9 at the neutron and gamma-ray irradiation for CHO-K1, and 0.3 at the neutron for T98G cells. D0 difference between 20 cGy/min and 80 cGy/min irradiation for T98G cells were 1.2 and 0.6 at neutron irradiation plus BPA and gamma-ray. The differences between neutron irradiations at the dose rate in T98G cells were supported by not only the cell viability but also 53BP1 foci assay at 24 h following irradiation to monitor DNA-DSBs. [Conclusion]Dose-rate effect of BNCT when T98G cells include 20 ppm BPA was greater than that of gamma-ray irradiation. Moreover, Dose-rate effect of the neutron beam when CHO-K1 cells did not include BPA was less than that of gamma-ray irradiation These present results may suggest the importance of dose-rate effect for more efficient BNCT and the side effect reduction. |
著作権等: | © 2013 Elsevier Ltd. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/188913 |
DOI(出版社版): | 10.1016/j.apradiso.2013.11.117 |
PubMed ID: | 24360864 |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このリポジトリに保管されているアイテムはすべて著作権により保護されています。