このアイテムのアクセス数: 111

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
humc.2013.066.pdf1.26 MBAdobe PDF見る/開く
タイトル: Long-term elimination of hepatitis C virus from human hepatocyte chimeric mice after interferon-γ gene transfer.
著者: Takahashi, Yuki  kyouindb  KAKEN_id
Ando, Mitsuru
Nishikawa, Makiya
Hiraga, Nobuhiko
Imamura, Michio
Chayama, Kazuaki
Takakura, Yoshinobu  kyouindb  KAKEN_id
著者名の別形: 高橋, 有己
高倉, 喜信
発行日: Mar-2014
出版者: Mary Ann Liebert, Inc.
誌名: Human gene therapy. Clinical development
巻: 25
号: 1
開始ページ: 28
終了ページ: 39
抄録: Chronic hepatitis C virus (HCV) infection is a leading cause of cirrhosis, liver failure, and hepatocellular carcinoma. Although the combination therapy employing pegylated interferon (IFN)-α and ribavirin is effective, this treatment is effective in only approximately 50% patients with genotype 1 HCV infection. IFN-γ is a potent anti-HCV agent that exhibits its antiviral action through a receptor distinct from that for IFN-α. Therefore, IFN-γ application might provide an alternative approach to IFN-α-based therapies. However, recombinant IFN-γ protein exhibits a poor pharmacokinetic property, that is, a very short half-life. It is our hypothesis that sustained IFN-γ serum concentrations produced by gene transfer could effectively eliminate HCV in vivo. We examined the in vivo antiviral activity in human hepatocyte chimeric mice infected with genotype 1b HCV at high HCV RNA titers (10(5)-10(7) copies/ml). The human IFN-γ-expressing plasmid vector pCpG-huIFNγ exhibited prolonged transgene expression in mice compared with the plasmid vector pCMV-huIFNγ. Moreover, the gene transfer of pCpG-huIFNγ eliminated HCV from the liver of the chimeric mice for a sustained period. On the contrary, administration of pCMV-huIFNγ could not eliminate HCV. In conclusion, we found that a single pCpG-huIFNγ injection resulted in long-term elimination of HCV RNA in chimeric mice, providing, for the first time, direct evidence that chronic infection with high titer HCV in vivo can be treated by sustained IFN-γ treatment.
著作権等: © 2013 Mary Ann Liebert, Inc.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/189317
DOI(出版社版): 10.1089/humc.2013.066
PubMed ID: 24279674
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。