ダウンロード数: 443
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.neulet.2014.07.025.pdf | 1.07 MB | Adobe PDF | 見る/開く |
タイトル: | Hydrogen protects auditory hair cells from cisplatin-induced free radicals. |
著者: | Kikkawa, Yayoi S Nakagawa, Takayuki Taniguchi, Mirei Ito, Juichi |
著者名の別形: | 中川, 隆之 |
キーワード: | Reactive oxygen species Hydroxyl radical Cochlea Hearing loss Antioxidant Chemotherapy |
発行日: | 5-Sep-2014 |
出版者: | Elsevier Ireland Ltd. |
誌名: | Neuroscience letters |
巻: | 579 |
開始ページ: | 125 |
終了ページ: | 129 |
抄録: | Cisplatin is a widely used chemotherapeutic agent for the treatment of various malignancies. However, its maximum dose is often limited by severe ototoxicity. Cisplatin ototoxicity may require the production of reactive oxygen species (ROS) in the inner ear by activating enzymes specific to the cochlea. Molecular hydrogen was recently established as an antioxidant that selectively reduces ROS, and has been reported to protect the central nervous system, liver, kidney and cochlea from oxidative stress. The purpose of this study was to evaluate the potential of molecular hydrogen to protect cochleae against cisplatin. We cultured mouse cochlear explants in medium containing various concentrations of cisplatin and examined the effects of hydrogen gas dissolved directly into the media. Following 48-h incubation, the presence of intact auditory hair cells was assayed by phalloidin staining. Cisplatin caused hair cell loss in a dose-dependent manner, whereas the addition of hydrogen gas significantly increased the numbers of remaining auditory hair cells. Additionally, hydroxyphenyl fluorescein (HPF) staining of the spiral ganglion showed that formation of hydroxyl radicals was successfully reduced in hydrogen-treated cochleae. These data suggest that molecular hydrogen can protect auditory tissues against cisplatin toxicity, thus providing an additional strategy to protect against drug-induced inner ear damage. |
著作権等: | © 2014 Elsevier Ireland Ltd. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/189409 |
DOI(出版社版): | 10.1016/j.neulet.2014.07.025 |
PubMed ID: | 25064701 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。