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Title: Preparation of immunostimulatory single-walled carbon nanotube/CpG DNA complexes and evaluation of their potential in cancer immunotherapy.
Authors: Zhou, Shuwen
Hashida, Yasuhiko
Kawakami, Shigeru
Mihara, Junya
Umeyama, Tomokazu  kyouindb  KAKEN_id  orcid (unconfirmed)
Imahori, Hiroshi  kyouindb  KAKEN_id
Murakami, Tatsuya
Yamashita, Fumiyoshi  kyouindb  KAKEN_id  orcid (unconfirmed)
Hashida, Mitsuru  kyouindb  KAKEN_id
Author's alias: 橋田, 充
Keywords: Carbon nanotubes
Immune activation
Issue Date: 25-Aug-2014
Publisher: Elsevier B.V.
Journal title: International journal of pharmaceutics
Volume: 471
Issue: 1-2
Start page: 214
End page: 223
Abstract: Carbon nanotubes (CNTs) have many interesting properties. In particular, their photohyperthermic effect by near-infrared (NIR) irradiation could be used to kill cancer cells, and could thus be applied in photohyperthermic therapy. However, the solubility of CNTs must be improved before they can be used in biological applications. As DNA is reported to disperse the CNTs in aqueous solution with π-π interactions, we hypothesis that immunostimulatory CpG DNA may also disperse the CNTs in aqueous solution. In this study, we used CpG DNA to disperse single-walled CNTs (SWCNTs) in aqueous solution, in order to combine photohyperthermic effect and immunoactivation together to achieve a more effective cancer therapy. As expected, CpG DNA effectively dispersed the SWCNTs in aqueous solution via the formation of SWCNT/CpG DNA complexes. Moreover, the immunoreactivity of the SWCNT/CpG DNA complexes was investigated. The results showed that intratumoral administration of the SWCNT/CpG DNA complexes in mice enhanced the production level of inflammatory cytokines in tumor tissues. Finally, we evaluated the antitumor effects of the SWCNT/CpG DNA complexes in tumor-bearing mice. The result indicated that intratumoral administration of the SWCNT/CpG DNA complexes combined with NIR irradiation was a more effective approach to prevent the proliferation of tumor growth.
Rights: © 2014 Elsevier B.V.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.ijpharm.2014.05.037
PubMed ID: 24861942
Appears in Collections:Journal Articles

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