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Title: LjMATE1: a citrate transporter responsible for iron supply to the nodule infection zone of Lotus japonicus.
Authors: Takanashi, Kojiro
Yokosho, Kengo
Saeki, Kazuhiko
Sugiyama, Akifumi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9643-6639 (unconfirmed)
Sato, Shusei
Tabata, Satoshi
Ma, Jian Feng
Yazaki, Kazufumi  kyouindb  KAKEN_id
Author's alias: 矢崎, 一史
Keywords: Citrate
Fe translocation
Lotus japonicus
MATE
Nodulation
Issue Date: Apr-2013
Publisher: Oxford University Press
Journal title: Plant & Cell Physiology
Volume: 54
Issue: 4
Start page: 585
End page: 594
Abstract: Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.
Rights: This is a pre-copyedited, author-produced PDF of an article accepted for publication in "Plant and cell physiology" following peer review. The version of record "Kojiro Takanashi, Kengo Yokosho, Kazuhiko Saeki, Akifumi Sugiyama, Shusei Sato, Satoshi Tabata, Jian Feng Ma, and Kazufumi Yazaki; LjMATE1: A Citrate Transporter Responsible for Iron Supply to the Nodule Infection Zone of Lotus japonicus; Plant Cell Physiol (2013) 54 (4): 585-594 first published online February 5, 2013 doi:10.1093/pcp/pct019" is available online at: http://pcp.oxfordjournals.org/content/54/4/585
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/189752
DOI(Published Version): 10.1093/pcp/pct019
PubMed ID: 23385147
Appears in Collections:Journal Articles

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