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Title: Salicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles.
Authors: Serizawa, Yasuhiro
Oshima, Rieko
Yoshida, Mitsuki
Sakon, Ichika
Kitani, Kazuto
Goto, Ayumi
Tsuda, Satoshi
Hayashi, Tatsuya  kyouindb  KAKEN_id
Author's alias: 大島, 里詠子
Keywords: Salicylate
5′-AMP-activated protein kinase
Glucose transport
Insulin signaling
Skeletal muscle
Diabetes mellitus
Issue Date: 10-Oct-2014
Publisher: Elsevier Inc.
Journal title: Biochemical and biophysical research communications
Volume: 453
Issue: 1
Start page: 81
End page: 85
Abstract: Salicylate (SAL) has been recently implicated in the antidiabetic effect in humans. We assessed whether 5'-AMP-activated protein kinase (AMPK) in skeletal muscle is involved in the effect of SAL on glucose homeostasis. Rat fast-twitch epitrochlearis and slow-twitch soleus muscles were incubated in buffer containing SAL. Intracellular concentrations of SAL increased rapidly (<5min) in both skeletal muscles, and the Thr(172) phosphorylation of the α subunit of AMPK increased in a dose- and time-dependent manner. SAL increased both AMPKα1 and AMPKα2 activities. These increases in enzyme activity were accompanied by an increase in the activity of 3-O-methyl-d-glucose transport, and decreases in ATP, phosphocreatine, and glycogen contents. SAL did not change the phosphorylation of insulin receptor signaling including insulin receptor substrate 1, Akt, and p70 ribosomal protein S6 kinase. These results suggest that SAL may be transported into skeletal muscle and may stimulate AMPK and glucose transport via energy deprivation in multiple muscle types. Skeletal muscle AMPK might be part of the mechanism responsible for the metabolic improvement induced by SAL.
Rights: © 2014 Elsevier Inc.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/192273
DOI(Published Version): 10.1016/j.bbrc.2014.09.066
PubMed ID: 25256746
Appears in Collections:Journal Articles

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