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Title: Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin.
Authors: Hiratsuka, Toru
Fujita, Yoshihisa  kyouindb  KAKEN_id
Honda, Naoki
Aoki, Kazuhiro
Kamioka, Yuji
Matsuda, Michiyuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 平塚, 徹
松田, 道行
Issue Date: 9-Feb-2015
Publisher: eLife Sciences Publications Ltd.
Journal title: eLife
Volume: 4
Thesis number: e05178
Abstract: Extracellular signal-regulated kinase (ERK) is a key effector of many growth signalling pathways. In this study, we visualise epidermal ERK activity in living mice using an ERK FRET biosensor. Under steady-state conditions, the epidermis occasionally revealed bursts of ERK activation patterns where ERK activity radially propagated from cell to cell. The frequency of this spatial propagation of radial ERK activity distribution (SPREAD) correlated with the rate of epidermal cell division. SPREADs and proliferation were stimulated by 12-O-tetradecanoylphorbol 13-acetate (TPA) in a manner dependent on EGF receptors and their cognate ligands. At the wounded skin, ERK activation propagated as trigger wave in parallel to the wound edge, suggesting that ERK activation propagation can be superimposed. Furthermore, by visualising the cell cycle, we found that SPREADs were associated with G2/M cell cycle progression. Our results provide new insights into how cell proliferation and transient ERK activity are synchronised in a living tissue.
Description: 皮膚の細胞増殖シグナルは打ち上げ花火のように伝搬する -表皮過形成性疾患の研究に新たな光-.京都大学プレスリリース. 2015-02-09.
Rights: Copyright Hiratsuka et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI(Published Version): 10.7554/eLife.05178
PubMed ID: 25668746
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