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j.bmc.2014.12.025.pdf | 647.66 kB | Adobe PDF | 見る/開く |
タイトル: | Rational design of specific binding hairpin Py-Im polyamides targeting human telomere sequences. |
著者: | Guo, Chuanxin Kawamoto, Yusuke ![]() ![]() ![]() Asamitsu, Sefan Sawatani, Yoshito Hashiya, Kaori Bando, Toshikazu ![]() ![]() Sugiyama, Hiroshi ![]() ![]() ![]() |
著者名の別形: | 杉山, 弘 |
キーワード: | Pyrrole–imidazole polyamide Telomeres DNA minor groove binder Melting temperature Surface plasmon resonance |
発行日: | 15-Feb-2015 |
出版者: | Elsevier Ltd. |
誌名: | Bioorganic & medicinal chemistry |
巻: | 23 |
号: | 4 |
開始ページ: | 855 |
終了ページ: | 860 |
抄録: | N-Methylpyrrole (Py)–N-methylimidazole (Im) polyamides are organic molecules that can recognize predetermined DNA sequences in a sequence-specific manner. Human telomeres contain regions of (TTAGGG)n repetitive nucleotide sequences at each end of chromosomes, and these regions protect the chromosome from deterioration or from fusion with neighboring chromosomes. The telomeres are disposable buffers at the ends of chromosomes that are truncated during cell division. Tandem hairpin Py–Im polyamide TH59, which recognizes human telomere sequences, was reported by Laemmli’s group in 2001. Here, we synthesized three types of Py–Im polyamides 1–3 based on TH59 for specific recognition of human telomere repeat sequences. Thermal melting temperature (Tm) measurements and surface plasmon resonance analysis were used to evaluate the abilities of the three types of Py–Im polyamides to discriminate between three kinds of DNA sequences. Significantly, the results showed that polyamides 1 and 2 have better affinities to TTAAGG than to TTAGGG. In contrast, polyamide 3 displayed good specificity to human telomere sequence, TTAGGG, as expected on the basis of Py–Im binding rules |
著作権等: | © 2015 Published by Elsevier Ltd. NOTICE: this is the author’s version of a work that was accepted for publication in Bioorganic & medicinal chemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Bioorganic & medicinal chemistry, 23(4), 2015, doi:10.1016/j.bmc.2014.12.025 この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/196038 |
DOI(出版社版): | 10.1016/j.bmc.2014.12.025 |
PubMed ID: | 25614111 |
出現コレクション: | 学術雑誌掲載論文等 |
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