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Title: Molecular Characterization of Hap Complex Components Responsible for Methanol-Inducible Gene Expression in the Methylotrophic Yeast Candida boidinii.
Authors: Oda, Saori
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7506-6184 (unconfirmed)
Nitta, Nobuhisa
Sasano, Yu
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Author's alias: 阪井, 康能
Issue Date: Mar-2015
Publisher: American Society for Microbiology
Journal title: Eukaryotic cell
Volume: 14
Issue: 3
Start page: 278
End page: 285
Abstract: We identified genes encoding components of the Hap complex, CbHAP2, CbHAP3, and CbHAP5, as transcription factors regulating methanol-inducible gene expression in the methylotrophic yeast Candida boidinii. We found that the Cbhap2Δ, Cbhap3Δ, and Cbhap5Δ gene-disrupted strains showed severe growth defects on methanol but not on glucose and nonfermentable carbon sources such as ethanol and glycerol. In these disruptants, the transcriptional activities of methanol-inducible promoters were significantly decreased compared to those of the wild-type strain, indicating that CbHap2p, CbHap3p, and CbHap5p play indispensable roles in methanol-inducible gene expression. Further molecular and biochemical analyses demonstrated that CbHap2p, CbHap3p, and CbHap5p localized to the nucleus and bound to the promoter regions of methanol-inducible genes regardless of the carbon source, and heterotrimer formation was suggested to be necessary for binding to DNA. Unexpectedly, distinct from Saccharomyces cerevisiae, the Hap complex functioned in methanol-specific induction rather than glucose derepression in C. boidinii. Our results shed light on a novel function of the Hap complex in methanol-inducible gene expression in methylotrophic yeasts.
Rights: Copyright © American Society for Microbiology, Eukaryotic cell, 14, 2015, 278–285, oi:10.1128/EC.00285-14.
URI: http://hdl.handle.net/2433/197211
DOI(Published Version): 10.1128/EC.00285-14
PubMed ID: 25595445
Appears in Collections:Journal Articles

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