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タイトル: | Selective isolation of β-glucan from corn pericarp hemicelluloses by affinity chromatography on cellulose column. |
著者: | Yoshida, Tomoki Honda, Yoichi https://orcid.org/0000-0002-7218-7367 (unconfirmed) Tsujimoto, Takashi Uyama, Hiroshi Azuma, Jun-ichi |
著者名の別形: | 吉田, 有希 |
キーワード: | β-Glucan Corn pericarp hemicelluloses Cellulose column Affinity chromatography |
発行日: | 13-Oct-2014 |
出版者: | Elsevier Ltd. |
誌名: | Carbohydrate polymers |
巻: | 111 |
開始ページ: | 538 |
終了ページ: | 542 |
抄録: | A combination of anion-exchange chromatography and affinity chromatography on a cellulose column was found to be effective for the isolation of β-(1, 3;1, 4)-glucan (BG) from corn pericarp hemicelluloses (CPHs). CPHs containing 6.6% BG were extracted from corn pericarp with 6M urea-2 wt% NaOH solution and initially fractionated into neutral and acidic parts by anion exchange chromatography to remove acidic arabinoxylan consisting of arabinose (35.6%) and xylose (50.9%). The neutral fraction (yield; 10.1% on the basis of CPHs) consisting of 1.0% arabinose, 10.1% xylose and 80.3% glucose containing 28.4% BG was then applied to a cellulose column of Whatman CF-11. BG could be recovered from the adsorbed fraction on the cellulose column by elution with 2% NaOH in a yield of 2.6% on the basis of CPHs with a purity of 84.7%. The chemical structure of the isolated corn pericarp BG was confirmed by (13)C NMR spectroscopic, methylation and lichenase treatment analyses. The results indicate that the ratios of (1, 4)/(1, 3) linkage and cellotriosyl/cellotetraosyl segments of the BG were 2.60 and 2.5, respectively. |
著作権等: | © 2014 Elsevier Ltd. NOTICE: this is the author's version of a work that was accepted for publication in Carbohydrate polymers. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate polymers, 113 538-542 (2014), doi:10.1016/j.carbpol.2014.04.050 This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/197293 |
DOI(出版社版): | 10.1016/j.carbpol.2014.04.050 |
PubMed ID: | 25037385 |
出現コレクション: | 学術雑誌掲載論文等 |
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