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Title: Selective isolation of β-glucan from corn pericarp hemicelluloses by affinity chromatography on cellulose column.
Authors: Yoshida, Tomoki  kyouindb  KAKEN_id
Honda, Yoichi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7218-7367 (unconfirmed)
Tsujimoto, Takashi
Uyama, Hiroshi
Azuma, Jun-ichi
Author's alias: 吉田, 有希
Keywords: β-Glucan
Corn pericarp hemicelluloses
Cellulose column
Affinity chromatography
Issue Date: 13-Oct-2014
Publisher: Elsevier Ltd.
Journal title: Carbohydrate polymers
Volume: 111
Start page: 538
End page: 542
Abstract: A combination of anion-exchange chromatography and affinity chromatography on a cellulose column was found to be effective for the isolation of β-(1, 3;1, 4)-glucan (BG) from corn pericarp hemicelluloses (CPHs). CPHs containing 6.6% BG were extracted from corn pericarp with 6M urea-2 wt% NaOH solution and initially fractionated into neutral and acidic parts by anion exchange chromatography to remove acidic arabinoxylan consisting of arabinose (35.6%) and xylose (50.9%). The neutral fraction (yield; 10.1% on the basis of CPHs) consisting of 1.0% arabinose, 10.1% xylose and 80.3% glucose containing 28.4% BG was then applied to a cellulose column of Whatman CF-11. BG could be recovered from the adsorbed fraction on the cellulose column by elution with 2% NaOH in a yield of 2.6% on the basis of CPHs with a purity of 84.7%. The chemical structure of the isolated corn pericarp BG was confirmed by (13)C NMR spectroscopic, methylation and lichenase treatment analyses. The results indicate that the ratios of (1, 4)/(1, 3) linkage and cellotriosyl/cellotetraosyl segments of the BG were 2.60 and 2.5, respectively.
Rights: © 2014 Elsevier Ltd. NOTICE: this is the author's version of a work that was accepted for publication in Carbohydrate polymers. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate polymers, 113 538-542 (2014), doi:10.1016/j.carbpol.2014.04.050
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/197293
DOI(Published Version): 10.1016/j.carbpol.2014.04.050
PubMed ID: 25037385
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