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ファイル | 記述 | サイズ | フォーマット | |
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j.1365-2443.2011.01520.x.pdf | 8.05 MB | Adobe PDF | 見る/開く |
タイトル: | Essential roles of Xenopus TRF2 in telomere end protection and replication. |
著者: | Muraki, Keiko Nabetani, Akira Nishiyama, Atsuya Ishikawa, Fuyuki https://orcid.org/0000-0002-5580-2305 (unconfirmed) |
著者名の別形: | 石川, 冬木 |
発行日: | 9-May-2011 |
出版者: | wiley |
誌名: | Genes to cells : devoted to molecular & cellular mechanisms |
巻: | 16 |
号: | 6 |
開始ページ: | 728 |
終了ページ: | 739 |
抄録: | TRF1 and TRF2 are double-stranded (ds) telomere DNA-binding proteins and the core members of shelterin, a complex that provides the structural and functional basis of telomere functions. We have reported that unlike mammalian TRF1 that constitutively binds to chromatin, Xenopus TRF1 (xTRF1) associates with mitotic chromatin but dissociates from interphase chromatin reconstituted in Xenopus egg extracts. This finding raised the possibility that xTRF1 and Xenopus TRF2 (xTRF2) contribute to telomere functions in a manner different from mammalian TRF1 and TRF2. Here, we focused on the role of xTRF2. We prepared chromatin reconstituted in egg extracts immunodepleted for xTRF2. Compared to mock-depleted nuclei, DNA damage response at telomeres was activated, and bulk DNAs were poorly replicated in xTRF2-depleted nuclei. The replication defect was rescued by inactivating ATR through the addition of anti-ATR neutralizing antibody, suggesting that ATR plays a role in the defect. Interestingly, the bulk DNA replication defect, but not the DNA damage response at telomeres, was rescued by supplementing the xTRF2-depleted extracts with recombinant xTRF2 (rTRF2). We propose that xTRF2 is required for both efficient replication of bulk DNA and protection from the activation of the DNA damage checkpoints pathway, and that those two functions are mechanistically separable. |
著作権等: | This is the peer reviewed version of the following article: Muraki, K., Nabetani, A., Nishiyama, A. and Ishikawa, F. (2011), Essential roles of Xenopus TRF2 in telomere end protection and replication. Genes to Cells, 16: 728–739, which has been published in final form at http://dx.doi.org/10.1111/j.1365-2443.2011.01520.x This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/197378 |
DOI(出版社版): | 10.1111/j.1365-2443.2011.01520.x |
PubMed ID: | 21554499 |
出現コレクション: | 学術雑誌掲載論文等 |
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