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Title: The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5'-CTGCAG-3'.
Authors: Shiraishi, Hideaki  kyouindb  KAKEN_id
Tabuse, Yosuke
Author's alias: 白石, 英秋
Keywords: Arthrospira platensis
Spirulina
restriction enzyme
DNA methyltransferase
edible cyanobacteria
Issue Date: 23-May-2013
Publisher: Taylor & Francis Group
Journal title: Bioscience, biotechnology, and biochemistry
Volume: 77
Issue: 4
Start page: 782
End page: 788
Abstract: The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5'-CTGCAG-3' between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper.
Rights: The Version of Record of this manuscript has been published and is available in Bioscience, Biotechnology, and Biochemistry (2013) http://www.tandfonline.com/10.1271/bbb.120919
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/197923
DOI(Published Version): 10.1271/bbb.120919
PubMed ID: 23563565
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