|Title:||The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5'-CTGCAG-3'.|
|Authors:||Shiraishi, Hideaki https://orcid.org/0000-0002-3490-9269 (unconfirmed)|
|Author's alias:||白石, 英秋|
|Publisher:||Taylor & Francis Group|
|Journal title:||Bioscience, biotechnology, and biochemistry|
|Abstract:||The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5'-CTGCAG-3' between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper.|
|Rights:||The Version of Record of this manuscript has been published and is available in Bioscience, Biotechnology, and Biochemistry (2013) http://www.tandfonline.com/10.1271/bbb.120919|
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|Appears in Collections:||Journal Articles |
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