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Title: | The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5'-CTGCAG-3'. |
Authors: | Shiraishi, Hideaki https://orcid.org/0000-0002-3490-9269 (unconfirmed) Tabuse, Yosuke |
Author's alias: | 白石, 英秋 |
Keywords: | Arthrospira platensis Spirulina restriction enzyme DNA methyltransferase edible cyanobacteria |
Issue Date: | 23-May-2013 |
Publisher: | Taylor & Francis Group |
Journal title: | Bioscience, biotechnology, and biochemistry |
Volume: | 77 |
Issue: | 4 |
Start page: | 782 |
End page: | 788 |
Abstract: | The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5'-CTGCAG-3' between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper. |
Rights: | The Version of Record of this manuscript has been published and is available in Bioscience, Biotechnology, and Biochemistry (2013) http://www.tandfonline.com/10.1271/bbb.120919 This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/197923 |
DOI(Published Version): | 10.1271/bbb.120919 |
PubMed ID: | 23563565 |
Appears in Collections: | Journal Articles |
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