Downloads: 130

Files in This Item:
File Description SizeFormat 
bbb.120919.pdf5.35 MBAdobe PDFView/Open
Title: The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5'-CTGCAG-3'.
Authors: Shiraishi, Hideaki  kyouindb  KAKEN_id  orcid (unconfirmed)
Tabuse, Yosuke
Author's alias: 白石, 英秋
Keywords: Arthrospira platensis
restriction enzyme
DNA methyltransferase
edible cyanobacteria
Issue Date: 23-May-2013
Publisher: Taylor & Francis Group
Journal title: Bioscience, biotechnology, and biochemistry
Volume: 77
Issue: 4
Start page: 782
End page: 788
Abstract: The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5'-CTGCAG-3' between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper.
Rights: The Version of Record of this manuscript has been published and is available in Bioscience, Biotechnology, and Biochemistry (2013)
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1271/bbb.120919
PubMed ID: 23563565
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks

Export Format: 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.