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dc.contributor.authorYamano, Takashien
dc.contributor.authorSato, Emien
dc.contributor.authorIguchi, Hiroen
dc.contributor.authorFukuda, Yurien
dc.contributor.authorFukuzawa, Hideyaen
dc.contributor.alternative山野, 隆志ja
dc.contributor.alternative福澤, 秀哉ja
dc.date.accessioned2015-06-02T05:04:40Z-
dc.date.available2015-06-02T05:04:40Z-
dc.date.issued2015-05-26-
dc.identifier.issn1091-6490-
dc.identifier.urihttp://hdl.handle.net/2433/198148-
dc.description藻類の光合成を支える二酸化炭素濃縮システムを解明. 京都大学プレスリリース. 2015-05-27.ja
dc.description.abstractThe supply of inorganic carbon (Ci; CO2 and HCO3 (-)) is an environmental rate-limiting factor in aquatic photosynthetic organisms. To overcome the difficulty in acquiring Ci in limiting-CO2 conditions, an active Ci uptake system called the CO2-concentrating mechanism (CCM) is induced to increase CO2 concentrations in the chloroplast stroma. An ATP-binding cassette transporter, HLA3, and a formate/nitrite transporter homolog, LCIA, are reported to be associated with HCO3 (-) uptake [Wang and Spalding (2014) Plant Physiol 166(4):2040-2050]. However, direct evidence of the route of HCO3 (-) uptake from the outside of cells to the chloroplast stroma remains elusive owing to a lack of information on HLA3 localization and comparative analyses of the contribution of HLA3 and LCIA to the CCM. In this study, we revealed that HLA3 and LCIA are localized to the plasma membrane and chloroplast envelope, respectively. Insertion mutants of HLA3 and/or LCIA showed decreased Ci affinities/accumulation, especially in alkaline conditions where HCO3 (-) is the predominant form of Ci. HLA3 and LCIA formed protein complexes independently, and the absence of LCIA decreased HLA3 mRNA accumulation, suggesting the presence of unidentified retrograde signals from the chloroplast to the nucleus to maintain HLA3 mRNA expression. Furthermore, although single overexpression of HLA3 or LCIA in high CO2 conditions did not affect Ci affinity, simultaneous overexpression of HLA3 with LCIA significantly increased Ci affinity/accumulation. These results highlight the HLA3/LCIA-driven cooperative uptake of HCO3 (-) and a key role of LCIA in the maintenance of HLA3 stability as well as Ci affinity/accumulation in the CCM.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherNational Academy of Sciencesen
dc.rights© 2015 National Academy of Sciencesen
dc.rightsこの論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.rightsThis is not the published version. Please cite only the published version.en
dc.subjectbicarbonate uptakeen
dc.subjectChlamydomonasen
dc.subjectchloroplast envelopeen
dc.subjectCO2-concentrating mechanismen
dc.subjectphotosynthesisen
dc.titleCharacterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii.en
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleProceedings of the National Academy of Sciences of the United States of Americaen
dc.identifier.volume112-
dc.identifier.issue23-
dc.identifier.epage7135-
dc.relation.doi10.1073/pnas.1501659112-
dc.textversionauthor-
dc.identifier.artnum7320-
dc.identifier.pmid26015566-
dc.relation.urlhttps://www.kyoto-u.ac.jp/ja/research-news/2015-05-27-
dcterms.accessRightsopen access-
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