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ファイル | 記述 | サイズ | フォーマット | |
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978-1-4939-1139-4_13.pdf | 866.38 kB | Adobe PDF | 見る/開く |
タイトル: | Visualization of Notch signaling oscillation in cells and tissues. |
著者: | Shimojo, Hiromi Harima, Yukiko Kageyama, Ryoichiro https://orcid.org/0000-0002-5985-1120 (unconfirmed) |
著者名の別形: | 影山, 龍一郎 |
キーワード: | Hes1 Hes7 Oscillatory expression Luciferase Bioluminescence CCD Neural stem cell Presomitic mesoderm Segmentation clock |
発行日: | 26-Jun-2014 |
出版者: | Springer New York |
誌名: | Methods in molecular biology |
巻: | 1187 |
開始ページ: | 169 |
終了ページ: | 179 |
抄録: | The Notch signaling effectors Hes1 and Hes7 exhibit oscillatory expression with a period of about 2-3 h during embryogenesis. Hes1 oscillation is important for proliferation and differentiation of neural stem cells, whereas Hes7 oscillation regulates periodic formation of somites. Continuous expression of Hes1 and Hes7 inhibits these developmental processes. Thus, expression dynamics are very important for gene functions, but it is difficult to distinguish between oscillatory and persistent expression by conventional methods such as in situ hybridization and immunostaining. Here, we describe time-lapse imaging methods using destabilized luciferase reporters and a highly sensitive cooled charge-coupled device camera, which can monitor dynamic gene expression. Furthermore, the expression of two genes can be examined simultaneously by a dual reporter system using two-color luciferase reporters. Time-lapse imaging analyses reveal how dynamically gene expression changes in many biological events. |
記述: | <Book Title>Notch Signaling |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-1139-4_13 許諾条件により本文ファイルは2015-06-26に公開. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/198460 |
DOI(出版社版): | 10.1007/978-1-4939-1139-4_13 |
PubMed ID: | 25053489 |
出現コレクション: | 学術雑誌掲載論文等 |
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