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Title: Multitarget super-resolution microscopy with high-density labeling by exchangeable probes.
Authors: Kiuchi, Tai  kyouindb  KAKEN_id
Higuchi, Makio
Takamura, Akihiro
Maruoka, Masahiro  kyouindb  KAKEN_id
Watanabe, Naoki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-8492-200X (unconfirmed)
Author's alias: 木内, 泰
Keywords: Super-resolution microscopy
Issue Date: 6-Jul-2015
Publisher: Nature Publishing Group
Journal title: Nature methods
Volume: 12
Start page: 743
End page: 746
Abstract: We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.
Description: 無制限多重染色と高精細な画質を実現する超解像顕微鏡法IRISを開発 -多種のタンパク質が織りなすミクロの世界を可視化-. 京都大学プレスリリース. 2015-07-07.
Rights: © 2015 Nature America, Inc.
許諾条件により本文ファイルは2016-01-06に公開.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/198729
DOI(Published Version): 10.1038/nmeth.3466
PubMed ID: 26147917
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2015-07-07
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