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Title: | Multitarget super-resolution microscopy with high-density labeling by exchangeable probes. |
Authors: | Kiuchi, Tai ![]() ![]() Higuchi, Makio Takamura, Akihiro Maruoka, Masahiro ![]() ![]() Watanabe, Naoki ![]() ![]() ![]() |
Author's alias: | 木内, 泰 |
Keywords: | Super-resolution microscopy |
Issue Date: | 6-Jul-2015 |
Publisher: | Nature Publishing Group |
Journal title: | Nature methods |
Volume: | 12 |
Start page: | 743 |
End page: | 746 |
Abstract: | We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell. |
Description: | 無制限多重染色と高精細な画質を実現する超解像顕微鏡法IRISを開発 -多種のタンパク質が織りなすミクロの世界を可視化-. 京都大学プレスリリース. 2015-07-07. |
Rights: | © 2015 Nature America, Inc. 許諾条件により本文ファイルは2016-01-06に公開. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/198729 |
DOI(Published Version): | 10.1038/nmeth.3466 |
PubMed ID: | 26147917 |
Related Link: | https://www.kyoto-u.ac.jp/ja/research-news/2015-07-07 |
Appears in Collections: | Journal Articles |

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