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Title: Downregulation of Pgc-1α expression by tea leaves and their by-products.
Authors: Shibuya, Erika
Murakami, Masaru
Kondo, Makoto
Kamei, Yasutomi
Tomonaga, Shozo  kyouindb  KAKEN_id  orcid (unconfirmed)
Matsui, Tohru
Funaba, Masayuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 舟場, 正幸
Keywords: Pgc-1α
skeletal muscle
tea leaf
muscle fibre type
Issue Date: 20-Sep-2013
Publisher: wiley
Journal title: Cell biochemistry and function
Volume: 32
Issue: 3
Start page: 236
End page: 240
Abstract: Previous studies indicate that muscle Pgc-1α expression governs the proportion of muscle fibre types. As a first step in using diet to manipulate the proportion of muscle fibre types by using Pgc-1α expression, the present study investigates the modulation of Pgc-1α expression by feedstuffs. A luciferase-based Pgc-1α reporter construct (Pgc-1α(-2553)-luc) that contains the mouse Pgc-1α promoter (-2553 to +78 bp) was prepared. A screen of ethanol extracts from 33 feedstuffs indicated that oolong tea and roasted green tea extracts decreased Pgc-1α(-2553)-luc expression in C2C12 myoblasts. The transcriptional repression of Pgc-1α by tea leaf extracts was reproduced in hepatic HepG2 cells. We further examined the effects of the alcohol extracts of tea waste and its silage on Pgc-1α transcription; the tea waste silage extract inhibited Pgc-1α transcription. Treatment with the extracts of raw tea leaves, tea waste and tea waste silage effectively decreased Pgc-1α mRNA levels during myogenesis of myosatellite cells. The present results suggest that tea leaves and their by-products could be used to modulate proportions of muscle fibre types.
Rights: This is the peer reviewed version of the following article: Shibuya, E., Murakami, M., Kondo, M., Kamei, Y., Tomonaga, S., Matsui, T. and Funaba, M. (2014), Downregulation of Pgc-1α expression by tea leaves and their by-products. Cell Biochem. Funct., 32: 236–240, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1002/cbf.3006
PubMed ID: 24114933
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