|Title:||Downregulation of Pgc-1α expression by tea leaves and their by-products.|
Tomonaga, Shozo https://orcid.org/0000-0003-0888-1407 (unconfirmed)
Funaba, Masayuki https://orcid.org/0000-0002-4303-6682 (unconfirmed)
|Author's alias:||舟場, 正幸|
muscle fibre type
|Journal title:||Cell biochemistry and function|
|Abstract:||Previous studies indicate that muscle Pgc-1α expression governs the proportion of muscle fibre types. As a first step in using diet to manipulate the proportion of muscle fibre types by using Pgc-1α expression, the present study investigates the modulation of Pgc-1α expression by feedstuffs. A luciferase-based Pgc-1α reporter construct (Pgc-1α(-2553)-luc) that contains the mouse Pgc-1α promoter (-2553 to +78 bp) was prepared. A screen of ethanol extracts from 33 feedstuffs indicated that oolong tea and roasted green tea extracts decreased Pgc-1α(-2553)-luc expression in C2C12 myoblasts. The transcriptional repression of Pgc-1α by tea leaf extracts was reproduced in hepatic HepG2 cells. We further examined the effects of the alcohol extracts of tea waste and its silage on Pgc-1α transcription; the tea waste silage extract inhibited Pgc-1α transcription. Treatment with the extracts of raw tea leaves, tea waste and tea waste silage effectively decreased Pgc-1α mRNA levels during myogenesis of myosatellite cells. The present results suggest that tea leaves and their by-products could be used to modulate proportions of muscle fibre types.|
|Rights:||This is the peer reviewed version of the following article: Shibuya, E., Murakami, M., Kondo, M., Kamei, Y., Tomonaga, S., Matsui, T. and Funaba, M. (2014), Downregulation of Pgc-1α expression by tea leaves and their by-products. Cell Biochem. Funct., 32: 236–240, which has been published in final form at http://dx.doi.org/10.1002/cbf.3006. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.|
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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