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タイトル: Selection and characterization of promoters based on genomic approach for the molecular breeding of oleaginous fungus Mortierella alpina 1S-4.
著者: Okuda, Tomoyo
Ando, Akinori  kyouindb  KAKEN_id
Sakuradani, Eiji
Kikukawa, Hiroshi
Kamada, Nozomu
Ochiai, Misa
Shima, Jun
Ogawa, Jun  kyouindb  KAKEN_id
著者名の別形: 奥田, 知生
小川, 順
キーワード: Mortierella alpina
Promoter
Expression sequence tag
Gene manipulation
発行日: 22-Feb-2014
出版者: Springer Berlin Heidelberg
誌名: Current genetics
巻: 60
号: 3
開始ページ: 183
終了ページ: 191
抄録: To express a foreign gene effectively, a good expression system is required. In this study, we investigated various promoters as useful tools for gene manipulation in oleaginous fungus Mortierella alpina 1S-4. We selected and cloned the promoter regions of 28 genes in M. alpina 1S-4 on the basis of expression sequence tag abundance data. The activity of each promoter was evaluated using the β-glucuronidase (GUS) reporter gene. Eight of these promoters were shown to enhance GUS expression more efficiently than a histone promoter, which is conventionally used for the gene manipulation in M. alpina. Especially, the predicted protein 3 and the predicted protein 6 promoters demonstrated approximately fivefold higher activity than the histone promoter. The activity of some promoters changed along with the cultivation phase of M. alpina 1S-4. Seven promoters with constitutive or time-dependent, high-level expression activity were selected, and deletion analysis was carried out to determine the promoter regions required to retain activity. This is the first report of comprehensive promoter analysis based on a genomic approach for M. alpina. The promoters described here will be useful tools for gene manipulation in this strain.
著作権等: The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-014-0423-4.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/198766
DOI(出版社版): 10.1007/s00294-014-0423-4
PubMed ID: 24562865
出現コレクション:学術雑誌掲載論文等

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