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タイトル: | Selection and characterization of promoters based on genomic approach for the molecular breeding of oleaginous fungus Mortierella alpina 1S-4. |
著者: | Okuda, Tomoyo Ando, Akinori https://orcid.org/0000-0002-8359-7441 (unconfirmed) Sakuradani, Eiji Kikukawa, Hiroshi Kamada, Nozomu Ochiai, Misa Shima, Jun Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) |
著者名の別形: | 奥田, 知生 小川, 順 |
キーワード: | Mortierella alpina Promoter Expression sequence tag Gene manipulation |
発行日: | 22-Feb-2014 |
出版者: | Springer Berlin Heidelberg |
誌名: | Current genetics |
巻: | 60 |
号: | 3 |
開始ページ: | 183 |
終了ページ: | 191 |
抄録: | To express a foreign gene effectively, a good expression system is required. In this study, we investigated various promoters as useful tools for gene manipulation in oleaginous fungus Mortierella alpina 1S-4. We selected and cloned the promoter regions of 28 genes in M. alpina 1S-4 on the basis of expression sequence tag abundance data. The activity of each promoter was evaluated using the β-glucuronidase (GUS) reporter gene. Eight of these promoters were shown to enhance GUS expression more efficiently than a histone promoter, which is conventionally used for the gene manipulation in M. alpina. Especially, the predicted protein 3 and the predicted protein 6 promoters demonstrated approximately fivefold higher activity than the histone promoter. The activity of some promoters changed along with the cultivation phase of M. alpina 1S-4. Seven promoters with constitutive or time-dependent, high-level expression activity were selected, and deletion analysis was carried out to determine the promoter regions required to retain activity. This is the first report of comprehensive promoter analysis based on a genomic approach for M. alpina. The promoters described here will be useful tools for gene manipulation in this strain. |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-014-0423-4. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/198766 |
DOI(出版社版): | 10.1007/s00294-014-0423-4 |
PubMed ID: | 24562865 |
出現コレクション: | 学術雑誌掲載論文等 |
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