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タイトル: Characterization of galactose-dependent promoters from an oleaginous fungus Mortierella alpina 1S-4.
著者: Okuda, Tomoyo
Ando, Akinori  kyouindb  KAKEN_id
Sakuradani, Eiji
Kikukawa, Hiroshi
Kamada, Nozomu
Ochiai, Misa
Shima, Jun
Ogawa, Jun  kyouindb  KAKEN_id
著者名の別形: 奥田, 知生
小川, 順
キーワード: Mortierella alpina
Inducible promoter
Galactose
Polyunsaturated fatty acid
Zygomycete
発行日: 19-Feb-2014
出版者: Springer Berlin Heidelberg
誌名: Current genetics
巻: 60
号: 3
開始ページ: 175
終了ページ: 182
抄録: An inducible promoter is a useful tool for the controlled expression of a given gene. In this report, we describe galactose-dependent promoters for potential use in an oleaginous fungus Mortierella alpina. We cloned the putative promoter regions of two genes encoding galactose metabolic enzymes, GAL1 and GAL10, from the genome of M. alpina 1S-4. The β-glucuronidase (GUS) reporter gene assay in M. alpina 1S-4 revealed that regulation of these promoters was dependent on the presence of galactose in the medium both with and without other sugars. With the GAL10 promoter, an approximately 50-fold increase of GUS activity was demonstrated by addition of galactose into the culture media at any cultivation phase. The 5' deletion analysis of the GAL10 promoter revealed that a promoter region of over 2, 000 bp length was required for its high-level activity and sufficient inducible response. Significantly, this is the first report of inducible promoters of zygomycetes. The GAL10 promoter will be a valuable tool for gene manipulation in M. alpina 1S-4.
著作権等: The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-014-0422-5.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/198767
DOI(出版社版): 10.1007/s00294-014-0422-5
PubMed ID: 24549368
出現コレクション:学術雑誌掲載論文等

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