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タイトル: | Characterization of galactose-dependent promoters from an oleaginous fungus Mortierella alpina 1S-4. |
著者: | Okuda, Tomoyo Ando, Akinori https://orcid.org/0000-0002-8359-7441 (unconfirmed) Sakuradani, Eiji Kikukawa, Hiroshi Kamada, Nozomu Ochiai, Misa Shima, Jun Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) |
著者名の別形: | 奥田, 知生 小川, 順 |
キーワード: | Mortierella alpina Inducible promoter Galactose Polyunsaturated fatty acid Zygomycete |
発行日: | 19-Feb-2014 |
出版者: | Springer Berlin Heidelberg |
誌名: | Current genetics |
巻: | 60 |
号: | 3 |
開始ページ: | 175 |
終了ページ: | 182 |
抄録: | An inducible promoter is a useful tool for the controlled expression of a given gene. In this report, we describe galactose-dependent promoters for potential use in an oleaginous fungus Mortierella alpina. We cloned the putative promoter regions of two genes encoding galactose metabolic enzymes, GAL1 and GAL10, from the genome of M. alpina 1S-4. The β-glucuronidase (GUS) reporter gene assay in M. alpina 1S-4 revealed that regulation of these promoters was dependent on the presence of galactose in the medium both with and without other sugars. With the GAL10 promoter, an approximately 50-fold increase of GUS activity was demonstrated by addition of galactose into the culture media at any cultivation phase. The 5' deletion analysis of the GAL10 promoter revealed that a promoter region of over 2, 000 bp length was required for its high-level activity and sufficient inducible response. Significantly, this is the first report of inducible promoters of zygomycetes. The GAL10 promoter will be a valuable tool for gene manipulation in M. alpina 1S-4. |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-014-0422-5. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/198767 |
DOI(出版社版): | 10.1007/s00294-014-0422-5 |
PubMed ID: | 24549368 |
出現コレクション: | 学術雑誌掲載論文等 |
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