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タイトル: | Akirin2 is critical for inducing inflammatory genes by bridging IκB-ζ and the SWI/SNF complex. |
著者: | Tartey, Sarang Matsushita, Kazufumi Vandenbon, Alexis https://orcid.org/0000-0003-2180-5732 (unconfirmed) Ori, Daisuke Imamura, Tomoko Mino, Takashi https://orcid.org/0000-0002-9562-008X (unconfirmed) Standley, Daron M Hoffmann, Jules A Reichhart, Jean-Marc Akira, Shizuo Takeuchi, Osamu https://orcid.org/0000-0002-1260-6232 (unconfirmed) |
著者名の別形: | 竹内, 理 |
発行日: | 16-Oct-2014 |
出版者: | EMBO press |
誌名: | The EMBO Journal |
巻: | 33 |
号: | 20 |
開始ページ: | 2332 |
終了ページ: | 2348 |
抄録: | Transcription of inflammatory genes in innate immune cells is coordinately regulated by transcription factors, including NF-κB, and chromatin modifiers. However, it remains unclear how microbial sensing initiates chromatin remodeling. Here, we show that Akirin2, an evolutionarily conserved nuclear protein, bridges NF-κB and the chromatin remodeling SWI/SNF complex by interacting with BRG1-Associated Factor 60 (BAF60) proteins as well as IκB-ζ, which forms a complex with the NF-κB p50 subunit. These interactions are essential for Toll-like receptor-, RIG-I-, and Listeria-mediated expression of proinflammatory genes including Il6 and Il12b in macrophages. Consistently, effective clearance of Listeria infection required Akirin2. Furthermore, Akirin2 and IκB-ζ recruitment to the Il6 promoter depend upon the presence of IκB-ζ and Akirin2, respectively, for regulation of chromatin remodeling. BAF60 proteins were also essential for the induction of Il6 in response to LPS stimulation. Collectively, the IκB-ζ-Akirin2-BAF60 complex physically links the NF-κB and SWI/SNF complexes in innate immune cell activation. By recruiting SWI/SNF chromatin remodellers to IκB-ζ, transcriptional coactivator for NF-κB, the conserved nuclear protein Akirin2 stimulates pro-inflammatory gene promoters in mouse macrophages during innate immune responses to viral or bacterial infection. |
著作権等: | © 2014 The Authors. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/199669 |
DOI(出版社版): | 10.15252/embj.201488447 |
PubMed ID: | 25107474 |
出現コレクション: | 学術雑誌掲載論文等 |
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