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Title: Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif.
Authors: Sanghamitra, Nusrat J M
Inaba, Hiroshi
Arisaka, Fumio
Ohtan Wang, Dan
Kanamaru, Shuji
Kitagawa, Susumu  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6956-9543 (unconfirmed)
Ueno, Takafumi
Author's alias: 稲葉, 央
北川, 進
Issue Date: 1-Aug-2014
Publisher: Royal Society of Chemistry
Journal title: Molecular BioSystems
Volume: 10
Issue: 10
Start page: 2677
End page: 2683
Abstract: Plasma membrane translocation is challenging due to the barrier of the cell membrane. Contrary to the synthetic cell-penetrating materials, tailed bacteriophages use cell-puncturing protein needles to puncture the cell membranes as an initial step of the DNA injection process. Cell-puncturing protein needles are thought to remain functional in the native phages. In this paper, we found that a bacteriophage T4 derived protein needle of 16 nm length spontaneously translocates through the living cell membrane. The β-helical protein needle (β-PN) internalizes into human red blood cells that lack endocytic machinery. By comparing the cellular uptake of β-PNs with modified surface charge, it is shown that the uptake efficiency is maximum when it has a negative charge corresponding to a zeta potential value of -16 mV. In HeLa cells, uptake of β-PN incorporates endocytosis independent mechanisms with partial macropinocytosis dependence. The endocytosis dependence of the uptake increases when the surface charges of β-PNs are modified to positive or negative. Thus, these results suggest that natural DNA injecting machinery can serve as an inspiration to design new class of cell-penetrating materials with a tailored mechanism.
Description: Accepted 25 Jul 2014.
Rights: This journal is © The Royal Society of Chemistry 2014.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/200240
DOI(Published Version): 10.1039/c4mb00293h
PubMed ID: 25082560
Appears in Collections:Journal Articles

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