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タイトル: | Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif. |
著者: | Sanghamitra, Nusrat J M Inaba, Hiroshi Arisaka, Fumio Ohtan Wang, Dan Kanamaru, Shuji Kitagawa, Susumu https://orcid.org/0000-0001-6956-9543 (unconfirmed) Ueno, Takafumi |
著者名の別形: | 稲葉, 央 北川, 進 |
発行日: | 1-Aug-2014 |
出版者: | Royal Society of Chemistry |
誌名: | Molecular BioSystems |
巻: | 10 |
号: | 10 |
開始ページ: | 2677 |
終了ページ: | 2683 |
抄録: | Plasma membrane translocation is challenging due to the barrier of the cell membrane. Contrary to the synthetic cell-penetrating materials, tailed bacteriophages use cell-puncturing protein needles to puncture the cell membranes as an initial step of the DNA injection process. Cell-puncturing protein needles are thought to remain functional in the native phages. In this paper, we found that a bacteriophage T4 derived protein needle of 16 nm length spontaneously translocates through the living cell membrane. The β-helical protein needle (β-PN) internalizes into human red blood cells that lack endocytic machinery. By comparing the cellular uptake of β-PNs with modified surface charge, it is shown that the uptake efficiency is maximum when it has a negative charge corresponding to a zeta potential value of -16 mV. In HeLa cells, uptake of β-PN incorporates endocytosis independent mechanisms with partial macropinocytosis dependence. The endocytosis dependence of the uptake increases when the surface charges of β-PNs are modified to positive or negative. Thus, these results suggest that natural DNA injecting machinery can serve as an inspiration to design new class of cell-penetrating materials with a tailored mechanism. |
記述: | Accepted 25 Jul 2014. |
著作権等: | This journal is © The Royal Society of Chemistry 2014. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/200240 |
DOI(出版社版): | 10.1039/c4mb00293h |
PubMed ID: | 25082560 |
出現コレクション: | 学術雑誌掲載論文等 |
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