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Title: Culture temperature affects redifferentiation and cartilaginous extracellular matrix formation in dedifferentiated human chondrocytes.
Authors: Ito, Akira  kyouindb  KAKEN_id  orcid (unconfirmed)
Aoyama, Tomoki  kyouindb  KAKEN_id
Iijima, Hirotaka
Tajino, Junichi
Nagai, Momoko
Yamaguchi, Shoki
Zhang, Xiangkai
Kuroki, Hiroshi  kyouindb  KAKEN_id
Author's alias: 伊藤, 明良
黒木, 裕士
Keywords: chondrocyte
extracellular matrix
pellet culture
Issue Date: 5-Mar-2015
Publisher: wiley
Journal title: Journal of orthopaedic research
Volume: 33
Issue: 5
Start page: 633
End page: 639
Abstract: To date, there have been few studies on how temperature affects the phenotype and metabolism of human chondrocytes. Thus, the purpose of this study was to elucidate the effects of culture temperature on chondrocyte redifferentiation and extracellular matrix (ECM) formation using dedifferentiated mature human chondrocytes in vitro. Dedifferentiated chondrocytes were cultured in a pellet culture system for up to 21 days. The pellets were randomly divided into three groups with different culture temperature (32, 37, and 41°C). Chondrocyte redifferentiation and ECM formation were evaluated by wet weight, messenger ribonucleic acid (mRNA), histological, and biochemical analyses. The results showed that the wet weight and the mRNA expressions of collagen type II A1 and cartilage oligomeric matrix protein at 37°C were higher than the corresponding values at 32°C. The histological and biochemical analyses revealed that the syntheses of type II collagen and proteoglycan were promoted at 37°C compared to those at 32°C, whereas they were considerably inhibited at 41°C. In conclusion, the results obtained herein indicated that temperature affects chondrocyte redifferentiation and ECM formation, and modulation of temperature might thus represent an advantageous means to regulate the phenotype and biosynthetic activity of chondrocytes.
Rights: This is the peer reviewed version of the following article: Ito, A., Aoyama, T., Iijima, H., Tajino, J., Nagai, M., Yamaguchi, S., Zhang, X. and Kuroki, H. (2015), Culture temperature affects redifferentiation and cartilaginous extracellular matrix formation in dedifferentiated human chondrocytes. J. Orthop. Res., 33: 633–639, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
The full-text file will be made open to the public on 5 MAR 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1002/jor.22808
PubMed ID: 25641400
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