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タイトル: | Locating the uracil-5-yl radical formed upon photoirradiation of 5-bromouracil-substituted DNA. |
著者: | Hashiya, Fumitaka Saha, Abhijit Kizaki, Seiichiro Li, Yue Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
著者名の別形: | 杉山, 弘 |
発行日: | 16-Dec-2014 |
出版者: | Oxford University Press |
誌名: | Nucleic acids research |
巻: | 42 |
号: | 22 |
開始ページ: | 13469 |
終了ページ: | 13473 |
抄録: | In a previous study, we found that 2-deoxyribonolactone is effectively generated in the specific 5-bromouracil ((Br)U)-substituted sequence 5'-(G/C)[A]n = 1,2 (Br)U(Br)U-3' and proposed that a formed uracil-5-yl radical mainly abstracts the C1' hydrogen from the 5'-side of (Br)U(Br)U under 302-nm irradiation condition. In the present work, we performed photoirradiation of (Br)U-substituted DNA in the presence of a hydrogen donor, tetrahydrofuran, to quench the uracil-5-yl radical to uracil and then subjected the sample to uracil DNA glycosylase digestion. Slab gel sequence analysis indicated that uracil residues were formed at the hot-spot sequence of 5'-(G/C)[A]n = 1,2 (Br)U(Br)U-3' in 302-nm irradiation of (Br)U-substituted DNA. Furthermore, we found that the uracil residue was also formed at the reverse sequence 5'-(Br)U(Br)U[A]n = 1,2(G/C)-3', which suggests that both 5'-(G/C)[A]n = 1,2 (Br)U(Br)U-3' and 5'-(Br)U(Br)U[A]n = 1,2(G/C)-3' are hot-spot sequences for the formation of the uracil-5-yl radical. |
著作権等: | © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/200779 |
DOI(出版社版): | 10.1093/nar/gku1133 |
PubMed ID: | 25398904 |
出現コレクション: | 学術雑誌掲載論文等 |
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