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タイトル: | Disruption of lig4 improves gene targeting efficiency in the oleaginous fungus Mortierella alpina 1S-4. |
著者: | Kikukawa, Hiroshi Sakuradani, Eiji Ando, Akinori https://orcid.org/0000-0002-8359-7441 (unconfirmed) Okuda, Tomoyo Ochiai, Misa Shimizu, Sakayu Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) |
著者名の別形: | 菊川, 寛史 小川, 順 |
キーワード: | DNA ligase 4 (lig4) Homologous recombination Gene targeting |
発行日: | 20-Aug-2015 |
出版者: | Elsevier B.V. |
誌名: | Journal of biotechnology |
巻: | 208 |
開始ページ: | 63 |
終了ページ: | 69 |
抄録: | The oil-producing zygomycete Mortierella alpina 1S-4 is known to accumulate beneficial polyunsaturated fatty acids. We identified the lig4 gene that encodes for a DNA ligase 4 homolog, which functions to repair double strand breaks by non-homologous end joining. We disrupted the lig4 gene to improve the gene targeting efficiency in M. alpina. The M. alpina 1S-4 Δlig4 strains showed no defect in vegetative growth, formation of spores, and fatty acid production, but exhibited high sensitivity to methyl methansulfonate, an agent that causes DNA double-strand breaks. Importantly, gene replacement of ura5 marker by CBXB marker occurred in 67% of Δlig4 strains and the gene targeting efficiency was 21-fold greater than that observed in disruption of the lig4 gene in the M. alpina 1S-4 host strain. Further metabolic engineering of the Δlig4 strains is expected to result in strains that produce higher levels of rare and beneficial polyunsaturated fatty acids and contribute to basic research on the zygomycete. |
著作権等: | © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ The full-text file will be made open to the public on 20 August 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/201593 |
DOI(出版社版): | 10.1016/j.jbiotec.2015.05.020 |
PubMed ID: | 26052021 |
出現コレクション: | 学術雑誌掲載論文等 |
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