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タイトル: | Hydration state inside HeLa cell monolayer investigated with terahertz spectroscopy |
著者: | Shiraga, K. Suzuki, T. Kondo, N. Tanaka, K. Ogawa, Y. |
著者名の別形: | 小川, 雄一 |
発行日: | 23-Jun-2015 |
出版者: | AIP Publishing |
誌名: | Applied Physics Letters |
巻: | 106 |
号: | 25 |
論文番号: | 253701 |
抄録: | The hydration state in living cells is believed to be associated with various cellular activities. Nevertheless, in vivo characterization of intracellular hydration state under physiological condition has not been well documented to date. In this study, the hydration state of an intact HeLa cell monolayer was investigated by terahertz time-domain attenuated total reflection spectroscopy. Combined with the extended theory of Onsager, we found 23.8 ± 7.4% of HeLa intracellular water was hydrated to biomolecules (corresponding to 1.25 g H[2]O/g solute); exhibiting slower relaxation dynamics than bulk water. |
著作権等: | © 2015 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. |
URI: | http://hdl.handle.net/2433/201614 |
DOI(出版社版): | 10.1063/1.4922918 |
出現コレクション: | 学術雑誌掲載論文等 |
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