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タイトル: | The active miniature inverted-repeat transposable element mPing posttranscriptionally produces new transcriptional variants in the rice genome |
著者: | Kum, Rise Tsukiyama, Takuji Inagaki, Haruka Saito, Hiroki Teraishi, Masayoshi https://orcid.org/0000-0002-6768-8052 (unconfirmed) Okumoto, Yutaka Tanisaka, Takatoshi |
著者名の別形: | 築山, 拓司 |
キーワード: | Rice Transposable element mPing Alternative splicing Alternative polyadenylation Posttranscriptional regulation |
発行日: | 18-Jul-2015 |
出版者: | Springer Netherlands |
誌名: | Molecular Breeding |
巻: | 35 |
号: | 8 |
論文番号: | 159 |
抄録: | Posttranscriptional RNA processing inclusive of alternative splicing and alternative polyadenylation, as well as transcriptional regulation, plays important regulatory roles in eukaryotic gene expression. In eukaryotic genomes, transposable elements can alter gene expression at both transcriptional and posttranscriptional levels. Miniature Ping (mPing) is an active miniature inverted-repeat TE discovered in the rice genome, and its insertion renders adjacent genes stress inducible. In this study, we examined the effect of mPing insertion into coding sequences on RNA processing. The 3′ RACE (rapid amplification of cDNA ends) analysis of mutant alleles, each harboring an mPing insertion, revealed that mPing induced various alternative splicing events. Furthermore, it was found that mPing induced alternative polyadenylation within its sequence. In the mutant allele, the body region of mPing was heavily methylated, whereas the mPing-flanking regions were moderately methylated. These results indicate that mPing alters transcript structures posttranscriptionally via induction of alternative splicing that most likely depends on DNA methylation. Based on these results, we discuss the availability of mPing as an insertional mutagen in rice. |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/s11032-015-0353-y. The full-text file will be made open to the public on 18 July 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/201985 |
DOI(出版社版): | 10.1007/s11032-015-0353-y |
出現コレクション: | 学術雑誌掲載論文等 |
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