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タイトル: | Gallium-68-labeled anti-HER2 single-chain Fv fragment: development and in vivo monitoring of HER2 expression. |
著者: | Ueda, Masashi Hisada, Hayato Temma, Takashi Shimizu, Yoichi Kimura, Hiroyuki Ono, Masahiro https://orcid.org/0000-0002-2497-039X (unconfirmed) Nakamoto, Yuji https://orcid.org/0000-0001-5783-8048 (unconfirmed) Togashi, Kaori Saji, Hideo |
著者名の別形: | 上田, 真史 佐治, 英郎 |
発行日: | Feb-2015 |
出版者: | Springer |
誌名: | Molecular imaging and biology |
巻: | 17 |
号: | 1 |
開始ページ: | 102 |
終了ページ: | 110 |
抄録: | 【Purpose】We aimed to develop a gallium-68 (Ga-68)-labeled single-chain variable fragment (scFv) targeting the human epidermal growth factor receptor 2 (HER2) to rapidly and noninvasively evaluate the status of HER2 expression. 【Procedures】Anti-HER2 scFv was labeled with Ga-68 by using deferoxamine (Df) as a bifunctional chelate. Biodistribution of [(68)Ga]Df-anti-HER2 scFv was examined with tumor-bearing mice and positron emission tomography (PET) imaging was performed. The changes in HER2 expression after anti-HER2 therapy were monitored by PET imaging. 【Results】[(68)Ga]Df-anti-HER2 scFv was obtained with high radiochemical yield after only a 5-min reaction at room temperature. The probe showed high accumulation in HER2-positive xenografts and the intratumoral distribution of radioactivity coincided with HER2-positive regions. Furthermore, [(68)Ga]Df-anti-HER2 scFv helped visualize HER2-positive xenografts and monitor the changes in HER2 expression after anti-HER2 therapy.【Conclusion】[(68)Ga]Df-anti-HER2 scFv could be a promising probe to evaluate HER2 status by in vivoPET imaging, unless trastuzumab is prescribed as part of the therapy. |
記述: | First online: 22 July 2014 |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/s11307-014-0769-5. The full-text file will be made open to the public on 22 July 2015 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/202106 |
DOI(出版社版): | 10.1007/s11307-014-0769-5 |
PubMed ID: | 25049073 |
出現コレクション: | 学術雑誌掲載論文等 |
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