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タイトル: Gallium-68-labeled anti-HER2 single-chain Fv fragment: development and in vivo monitoring of HER2 expression.
著者: Ueda, Masashi
Hisada, Hayato
Temma, Takashi
Shimizu, Yoichi  kyouindb  KAKEN_id
Kimura, Hiroyuki
Ono, Masahiro  kyouindb  KAKEN_id
Nakamoto, Yuji
Togashi, Kaori
Saji, Hideo
著者名の別形: 上田, 真史
佐治, 英郎
発行日: Feb-2015
出版者: Springer US
誌名: Molecular imaging and biology
巻: 17
号: 1
開始ページ: 102
終了ページ: 110
抄録: 【Purpose】We aimed to develop a gallium-68 (Ga-68)-labeled single-chain variable fragment (scFv) targeting the human epidermal growth factor receptor 2 (HER2) to rapidly and noninvasively evaluate the status of HER2 expression. 【Procedures】Anti-HER2 scFv was labeled with Ga-68 by using deferoxamine (Df) as a bifunctional chelate. Biodistribution of [(68)Ga]Df-anti-HER2 scFv was examined with tumor-bearing mice and positron emission tomography (PET) imaging was performed. The changes in HER2 expression after anti-HER2 therapy were monitored by PET imaging. 【Results】[(68)Ga]Df-anti-HER2 scFv was obtained with high radiochemical yield after only a 5-min reaction at room temperature. The probe showed high accumulation in HER2-positive xenografts and the intratumoral distribution of radioactivity coincided with HER2-positive regions. Furthermore, [(68)Ga]Df-anti-HER2 scFv helped visualize HER2-positive xenografts and monitor the changes in HER2 expression after anti-HER2 therapy.【Conclusion】[(68)Ga]Df-anti-HER2 scFv could be a promising probe to evaluate HER2 status by in vivoPET imaging, unless trastuzumab is prescribed as part of the therapy.
記述: First online: 22 July 2014
著作権等: The final publication is available at Springer via http://dx.doi.org/10.1007/s11307-014-0769-5.
The full-text file will be made open to the public on 22 July 2015 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/202106
DOI(出版社版): 10.1007/s11307-014-0769-5
PubMed ID: 25049073
出現コレクション:学術雑誌掲載論文等

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