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Title: Grease matrix as a versatile carrier of proteins for serial crystallography.
Authors: Sugahara, Michihiro
Mizohata, Eiichi
Nango, Eriko  kyouindb  KAKEN_id
Suzuki, Mamoru
Tanaka, Tomoyuki
Masuda, Tetsuya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5857-5753 (unconfirmed)
Tanaka, Rie
Shimamura, Tatsuro  kyouindb  KAKEN_id
Tanaka, Yoshiki
Suno, Chiyo
Ihara, Kentaro
Pan, Dongqing  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-3701-8442 (unconfirmed)
Kakinouchi, Keisuke
Sugiyama, Shigeru
Murata, Michio
Inoue, Tsuyoshi
Tono, Kensuke
Song, Changyong
Park, Jaehyun
Kameshima, Takashi
Hatsui, Takaki
Joti, Yasumasa
Yabashi, Makina
Iwata, So  kyouindb  KAKEN_id
Author's alias: 桝田, 哲哉
Keywords: Nanocrystallography
Proteins
Issue Date: 10-Nov-2014
Publisher: Nature Publishing Group
Journal title: Nature methods
Volume: 12
Issue: 1
Start page: 61
End page: 63
Abstract: Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
Description: 連続フェムト秒結晶構造解析のための結晶供給手法を開発 -少量の試料で多様なタンパク質の構造決定がSACLAで可能に-. 京都大学プレスリリース. 2014-11-11.
Rights: This is the manuscript version of the article is available at http://dx.doi.org/10.1038/nmeth.3172.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/203008
DOI(Published Version): 10.1038/nmeth.3172
PubMed ID: 25384243
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2014-11-11
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