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タイトル: | Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases. |
著者: | Ishida, Kentaro Gee, Peter Hotta, Akitsu https://orcid.org/0000-0002-2619-7441 (unconfirmed) |
著者名の別形: | 堀田, 秋津 |
キーワード: | CRISPR Cas9 genome editing mutagenesis off-target effect |
発行日: | 16-Oct-2015 |
出版者: | MDPI |
誌名: | International journal of molecular sciences |
巻: | 16 |
号: | 10 |
開始ページ: | 24751 |
終了ページ: | 24771 |
抄録: | Programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator like effector nucleases (TALENs), and clustered regularly interspersed short palindromic repeats associated protein-9 (CRISPR-Cas9), hold tremendous potential for applications in the clinical setting to treat genetic diseases or prevent infectious diseases. However, because the accuracy of DNA recognition by these nucleases is not always perfect, off-target mutagenesis may result in undesirable adverse events in treated patients such as cellular toxicity or tumorigenesis. Therefore, designing nucleases and analyzing their activity must be carefully evaluated to minimize off-target mutagenesis. Furthermore, rigorous genomic testing will be important to ensure the integrity of nuclease modified cells. In this review, we provide an overview of available nuclease designing platforms, nuclease engineering approaches to minimize off-target activity, and methods to evaluate both on- and off-target cleavage of CRISPR-Cas9. |
著作権等: | This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/203025 |
DOI(出版社版): | 10.3390/ijms161024751 |
PubMed ID: | 26501275 |
出現コレクション: | 学術雑誌掲載論文等 |
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