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タイトル: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion.
著者: Yata, Tomoya
Takahashi, Yuki  kyouindb  KAKEN_id
Tan, Mengmeng
Hidaka, Kumi
Sugiyama, Hiroshi
Endo, Masayuki  kyouindb  KAKEN_id
Takakura, Yoshinobu  kyouindb  KAKEN_id
Nishikawa, Makiya
著者名の別形: 高橋, 有己
西川, 元也
発行日: 14-Oct-2015
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 5
論文番号: 14979
抄録: The application of DNA as a functional material such as DNA hydrogel has attracted much attention. Despite an increasing interest, the high cost of DNA synthesis is a limiting factor for its utilization. To reduce the cost, we report here a highly efficient amplification technique for polypod-like structured DNA (polypodna) with adhesive ends that spontaneously forms DNA hydrogel. Two types of polypodna with three (tripodna) and four (tetrapodna) pods were selected, and a template oligodeoxynucleotide, containing a tandem sequence of a looped tripodna or tetrapodna, respectively, along with restriction enzyme (TspRI) sites, was designed. The template was circularized using T4 DNA ligase, and amplified by rolling circle amplification (RCA). The RCA product was highly viscous and resistant to restriction digestion. Observation under an electron microscope revealed microflower-like structures. These structures were composed of long DNA and magnesium pyrophosphate, and their treatment with EDTA followed by restriction digestion with TspRI resulted in numerous copies of polypodna with adhesive ends, which formed a DNA hydrogel. Thus, we believe this technique provides a new approach to produce DNA nanostructures, and helps in expanding their practical applications.
記述: [Corrigendum (07 January 2016)] Scientific Reports 6: Article number:17249; published online: 07 January 2016: doi:10.1038/srep17249
著作権等: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/203046
DOI(出版社版): 10.1038/srep14979
PubMed ID: 26462616
関連リンク: http://dx.doi.org/10.1038/srep17249
出現コレクション:学術雑誌掲載論文等

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