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タイトル: | Gene targeting in the oil-producing fungus Mortierella alpina 1S-4 and construction of a strain producing a valuable polyunsaturated fatty acid. |
著者: | Kikukawa, Hiroshi Sakuradani, Eiji Nakatani, Masato Ando, Akinori https://orcid.org/0000-0002-8359-7441 (unconfirmed) Okuda, Tomoyo Sakamoto, Takaiku Ochiai, Misa Shimizu, Sakayu Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) |
著者名の別形: | 菊川, 寛史 小川, 順 |
キーワード: | Mortierella alpina Ku80 Homologous recombination Gene targeting Δ5-desaturase Dihomo-γ-linolenic acid |
発行日: | Nov-2015 |
出版者: | Springer Berlin Heidelberg |
誌名: | Current genetics |
巻: | 61 |
号: | 4 |
開始ページ: | 579 |
終了ページ: | 589 |
抄録: | To develop an efficient gene-targeting system in Mortierella alpina 1S-4, we identified the ku80 gene encoding the Ku80 protein, which is involved in the nonhomologous end-joining pathway in genomic double-strand break (DSB) repair, and constructed ku80 gene-disrupted strains via single-crossover homologous recombination. The Δku80 strain from M. alpina 1S-4 showed no negative effects on vegetative growth, formation of spores, and fatty acid productivity, and exhibited high sensitivity to methyl methanesulfonate, which causes DSBs. Dihomo-γ-linolenic acid (DGLA)-producing strains were constructed by disruption of the Δ5-desaturase gene, encoding a key enzyme of bioconversion of DGLA to ARA, using the Δku80 strain as a host strain. The significant improvement of gene-targeting efficiency was not observed by disruption of the ku80 gene, but the construction of DGLA-producing strain by disruption of the Δ5-desaturase gene was succeeded using the Δku80 strain as a host strain. This report describes the first study on the identification and disruption of the ku80 gene in zygomycetes and construction of a DGLA-producing transformant using a gene-targeting system in M. alpina 1S-4. |
記述: | First online: 18 March 2015 |
著作権等: | The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-015-0481-2. The full-text file will be made open to the public on 18 March 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/203078 |
DOI(出版社版): | 10.1007/s00294-015-0481-2 |
PubMed ID: | 25782448 |
出現コレクション: | 学術雑誌掲載論文等 |
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