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タイトル: Gene targeting in the oil-producing fungus Mortierella alpina 1S-4 and construction of a strain producing a valuable polyunsaturated fatty acid.
著者: Kikukawa, Hiroshi
Sakuradani, Eiji
Nakatani, Masato
Ando, Akinori  kyouindb  KAKEN_id
Okuda, Tomoyo
Sakamoto, Takaiku
Ochiai, Misa
Shimizu, Sakayu
Ogawa, Jun  kyouindb  KAKEN_id
著者名の別形: 菊川, 寛史
小川, 順
キーワード: Mortierella alpina
Ku80
Homologous recombination
Gene targeting
Δ5-desaturase
Dihomo-γ-linolenic acid
発行日: Nov-2015
出版者: Springer Berlin Heidelberg
誌名: Current genetics
巻: 61
号: 4
開始ページ: 579
終了ページ: 589
抄録: To develop an efficient gene-targeting system in Mortierella alpina 1S-4, we identified the ku80 gene encoding the Ku80 protein, which is involved in the nonhomologous end-joining pathway in genomic double-strand break (DSB) repair, and constructed ku80 gene-disrupted strains via single-crossover homologous recombination. The Δku80 strain from M. alpina 1S-4 showed no negative effects on vegetative growth, formation of spores, and fatty acid productivity, and exhibited high sensitivity to methyl methanesulfonate, which causes DSBs. Dihomo-γ-linolenic acid (DGLA)-producing strains were constructed by disruption of the Δ5-desaturase gene, encoding a key enzyme of bioconversion of DGLA to ARA, using the Δku80 strain as a host strain. The significant improvement of gene-targeting efficiency was not observed by disruption of the ku80 gene, but the construction of DGLA-producing strain by disruption of the Δ5-desaturase gene was succeeded using the Δku80 strain as a host strain. This report describes the first study on the identification and disruption of the ku80 gene in zygomycetes and construction of a DGLA-producing transformant using a gene-targeting system in M. alpina 1S-4.
記述: First online: 18 March 2015
著作権等: The final publication is available at Springer via http://dx.doi.org/10.1007/s00294-015-0481-2.
The full-text file will be made open to the public on 18 March 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/203078
DOI(出版社版): 10.1007/s00294-015-0481-2
PubMed ID: 25782448
出現コレクション:学術雑誌掲載論文等

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