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タイトル: | A novel unsaturated fatty acid hydratase toward C(16) to C(22) fatty acids from Lactobacillus acidophilus. |
著者: | Hirata, Akiko Kishino, Shigenobu https://orcid.org/0000-0002-4587-2422 (unconfirmed) Park, Si-Bum Takeuchi, Michiki Kitamura, Nahoko Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) |
著者名の別形: | 岸野, 重信 小川, 順 |
キーワード: | arachidonic acid docosahexaenoic acid enzymology fatty acid/metabolism lipids lipids/chemistry lactic acid bacteria hydroxy fatty acid hydration dehydration |
発行日: | Jul-2015 |
出版者: | American Society for Biochemistry and Molecular Biology |
誌名: | Journal of lipid research |
巻: | 56 |
号: | 7 |
開始ページ: | 1340 |
終了ページ: | 1350 |
抄録: | Hydroxy FAs, one of the gut microbial metabolites of PUFAs, have attracted much attention because of their various bioactivities. The purpose of this study was to identify lactic acid bacteria with the ability to convert linoleic acid (LA) to hydroxy FAs. A screening process revealed that a gut bacterium, Lactobacillus acidophilus NTV001, converts LA mainly into 13-hydroxy-cis-9-octadecenoic acid and resulted in the identification of the hydratase responsible, fatty acid hydratase 1 (FA-HY1). Recombinant FA-HY1 was purified, and its enzymatic characteristics were investigated. FA-HY1 could convert not only C18 PUFAs but also C20 and C22 PUFAs. C18 PUFAs with a cis carbon-carbon double bond at the Δ12 position were converted into the corresponding 13-hydroxy FAs. Arachidonic acid and DHA were converted into the corresponding 15-hydroxy FA and 14-hydroxy FA, respectively. To the best of our knowledge, this is the first report of a bacterial FA hydratase that can convert C20 and C22 PUFAs into the corresponding hydroxy FAs. These novel hydroxy FAs produced by using FA-HY1 should contribute to elucidating the bioactivities of hydroxy FAs. |
著作権等: | This research was originally published in ['Journal of lipid research' July 2015, 56, 1340-1350.] © the American Society for Biochemistry and Molecular Biology この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/204520 |
DOI(出版社版): | 10.1194/jlr.M059444 |
PubMed ID: | 25966711 |
出現コレクション: | 学術雑誌掲載論文等 |
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