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Title: 5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability.
Authors: Kafer, Georgia Rose
Li, Xuan
Horii, Takuro
Suetake, Isao
Tajima, Shoji
Hatada, Izuho
Carlton, Peter Mark
Issue Date: 16-Feb-2016
Publisher: Elsevier Inc.
Journal title: Cell reports
Volume: 14
Issue: 6
Start page: 1283
End page: 1292
Abstract: 5-hydroxymethylcytosine (5hmC) is a DNA base created during active DNA demethylation by the recently discovered TET enzymes. 5hmC has essential roles in gene expression and differentiation. Here, we demonstrate that 5hmC also localizes to sites of DNA damage and repair. 5hmC accumulates at damage foci induced by aphidicolin and microirradiation and colocalizes with major DNA damage response proteins 53BP1 and γH2AX, revealing 5hmC as an epigenetic marker of DNA damage. Deficiency for the TET enzymes eliminates damage-induced 5hmC accumulation and elicits chromosome segregation defects in response to replication stress. Our results indicate that the TET enzymes and 5hmC play essential roles in ensuring genome integrity.
Rights: © 2016 The Authors. Published by Elsevier Inc.
This is an open access article under the CC BY license (
DOI(Published Version): 10.1016/j.celrep.2016.01.035
PubMed ID: 26854228
Appears in Collections:Journal Articles

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