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j.imlet.2015.10.008.pdf | 638.18 kB | Adobe PDF | 見る/開く |
タイトル: | In vivo and in vitro analyses of α-galactosylceramide uptake by conventional dendritic cell subsets using its fluorescence-labeled derivative. |
著者: | Ushida, Maki Iyoda, Tomonori Kanamori, Mitsuhiro Watarai, Hiroshi Takahara, Kazuhiko https://orcid.org/0000-0003-4730-8187 (unconfirmed) Inaba, Kayo |
著者名の別形: | 高原, 和彦 |
キーワード: | α-Galactosylceramide Dendritic cells IFN-γ iNKT cells Subsets |
発行日: | Dec-2015 |
出版者: | Elsevier B.V. |
誌名: | Immunology letters |
巻: | 168 |
号: | 2 |
開始ページ: | 300 |
終了ページ: | 305 |
抄録: | Conventional dendritic cells (cDCs) present α-galactosylceramide (αGC) to invariant natural killer T (iNKT) cells through CD1d. Among cDC subsets, CD8(+) DCs efficiently induce IFN-γ production in iNKT cells. Using fluorescence-labeled αGC, we showed that CD8(+) DCs incorporated larger amounts of αGC and kept it intact longer than CD8(-) DCs. Histological analyses revealed that Langerin(+)CD8(+) DCs in the splenic marginal zone, which was the unique equipment to capture blood-borne antigens, preferably incorporated αGC, and the depletion of Langerin(+) cells decreased IFN-γ and IL-12 production in response to αGC. Furthermore, splenic Langerin(+)CD8(+) DCs expressed more membrane-bound CXCL16, which possibly anchored iNKT cells in the marginal zone, than CD8(-) DCs. Collectively, it is suggested that the cellular properties and localization of CD8(+) DCs are important for stimulation of iNKT cells by αGC. |
著作権等: | © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ The full-text file will be made open to the public on 1 December 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/207429 |
DOI(出版社版): | 10.1016/j.imlet.2015.10.008 |
PubMed ID: | 26481266 |
出現コレクション: | 学術雑誌掲載論文等 |
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