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Title: Bidirectional effects of dexmedetomidine on human platelet functions in vitro.
Authors: Kawamoto, Shuji  kyouindb  KAKEN_id
Hirakata, Hideo
Sugita, Naoko
Fukuda, Kazuhiko  kyouindb  KAKEN_id
Author's alias: 川本, 修司
Keywords: Dexmedetomidine
Platelet
α[2]-Adrenoceptor
Imidazoline receptor
Issue Date: 5-Nov-2015
Publisher: Elsevier B.V.
Journal title: European journal of pharmacology
Volume: 766
Start page: 122
End page: 128
Abstract: Platelets express the imidazoline (I)-receptor, I[1] and I[2], as well as the α[2]-adrenoceptor. Although dexmedetomidine, a selective α[2]-adrenoceptor agonist with some affinity for the I-receptor is expected to affect platelet function, the effects of dexmedetomidine on platelet functions remain unclear. In the present study, we investigated the effects of dexmedetomidine on human platelet functions in vitro. The effects of dexmedetomidine on platelet aggregation were examined using aggregometers. The formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in platelets was measured by an enzyme immunoassay. In addition, P-selectin expression in platelets was estimated by flow cytometry. We showed that dexmedetomidine enhances platelet aggregation. But in the presence of yohimbine, an α[2]-antagonist, dexmedetomidine suppressed platelet aggregation. Efaroxan, an I[1]-antagonist, and methylene blue, a soluble guanylate cyclase inhibitor, abolished the suppressive effect of dexmedetomidine, whereas idazoxan, an I[2]-antagonist, showed no effect. Dexmedetomidine suppressed cAMP formation and enhanced P-selectin expression in platelets, and these effects were inhibited by yohimbine. Dexmedetomidine increased cGMP formation in platelets in the presence of yohimbine, and this increase was suppressed by efaroxan. These results demonstrated that dexmedetomidine has both enhancing and suppressive effects on human platelet functions through its action on the α[2]-adrenoceptor and on the I[1]-imidazoline receptor, respectively.
Rights: © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
The full-text file will be made open to the public on 5 December 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/207650
DOI(Published Version): 10.1016/j.ejphar.2015.09.049
PubMed ID: 26435028
Appears in Collections:Journal Articles

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