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タイトル: | Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. |
著者: | Ishizaki, Kimitsune Nishihama, Ryuichi https://orcid.org/0000-0002-7032-732X (unconfirmed) Ueda, Minoru Inoue, Keisuke Ishida, Sakiko Nishimura, Yoshiki https://orcid.org/0000-0001-8686-9206 (unconfirmed) Shikanai, Toshiharu Kohchi, Takayuki https://orcid.org/0000-0002-9712-4872 (unconfirmed) |
著者名の別形: | 河内, 孝之 |
発行日: | 25-Sep-2015 |
出版者: | Public Library of Science |
誌名: | PLOS ONE |
巻: | 10 |
号: | 9 |
論文番号: | e0138876 |
抄録: | We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha. |
著作権等: | © 2015 Ishizaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
URI: | http://hdl.handle.net/2433/207679 |
DOI(出版社版): | 10.1371/journal.pone.0138876 |
PubMed ID: | 26406247 |
出現コレクション: | 学術雑誌掲載論文等 |
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