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Title: Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.
Authors: Ishizaki, Kimitsune
Nishihama, Ryuichi  kyouindb  KAKEN_id  orcid (unconfirmed)
Ueda, Minoru
Inoue, Keisuke  kyouindb  KAKEN_id
Ishida, Sakiko
Nishimura, Yoshiki  kyouindb  KAKEN_id  orcid (unconfirmed)
Shikanai, Toshiharu  kyouindb  KAKEN_id
Kohchi, Takayuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 河内, 孝之
Issue Date: 25-Sep-2015
Publisher: Public Library of Science
Journal title: PLOS ONE
Volume: 10
Issue: 9
Thesis number: e0138876
Abstract: We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.
Rights: © 2015 Ishizaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
DOI(Published Version): 10.1371/journal.pone.0138876
PubMed ID: 26406247
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