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Title: Kinetoplastid flagellates overlooked by universal primers dominate in the oxygenated hypolimnion of Lake Biwa, Japan.
Authors: Mukherjee, Indranil
Hodoki, Yoshikuni  kyouindb  KAKEN_id
Nakano, Shin-Ichi  kyouindb  KAKEN_id
Author's alias: 中野, 伸一
Keywords: 18S rRNA gene
CARD-FISH
clone library
kinetoplastids
lakes
nanoflagellates
Issue Date: Aug-2015
Publisher: Oxford University Press
Journal title: FEMS microbiology ecology
Volume: 91
Issue: 8
Thesis number: fiv083
Abstract: Kinetoplastid flagellates, microscopically often detected from various aquatic environments and considered ubiquitous are seldom reported in molecular diversity studies with universal eukaryote DNA primers. To investigate this inconsistency, we examined nanoflagellate diversity in Lake Biwa, Japan by 18S rRNA gene clone libraries using universal eukaryote and kinetoplastid-specific primers. We also examined the abundance of kinetoplastids by Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization. No, kinetoplastid sequences were detected in the universal eukaryote primers library from epilimnion and hypolimnion in different seasons. However, kinetoplastid flagellates were detected with kinetoplastid-specific probe from all of the seasons and contributed up to 11.9 and 36.0% of total eukaryotes in the epilimnion and hypolimnion, respectively. Thus, kinetoplastids probably are a significant, sometimes dominant, group in the hypolimnion, contributing up to 43.7% of the total flagellates. Using group-specific primers, kinetoplastid sequences were also obtained from both epilimnion and hypolimnion library. Therefore, we attributed the inconsistency to the divergent nature of 18S rRNA gene of kinetoplastids, which lead to their undetection in the universal eukaryote primer libraries. This study revealed that kinetoplastids have significant ecological importance in the hypolimnion of Lake Biwa, suggesting that these flagellates have been overlooked in other studies using universal eukaryote primers.
Rights: This is a pre-copyedited, author-produced PDF of an article accepted for publication in 'FEMS microbiology ecology' following peer review. The version of record [Indranil Mukherjee, Yoshikuni Hodoki, Shin-ichi Nakano. Kinetoplastid flagellates overlooked by universal primers dominate in the oxygenated hypolimnion of Lake Biwa, Japan. Volume 91, Issue 8, fiv083, August 2015] is available online at: http://femsec.oxfordjournals.org/content/91/8/fiv083.
The full-text file will be made open to the public on 16 July 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/207708
DOI(Published Version): 10.1093/femsec/fiv083
PubMed ID: 26187480
Appears in Collections:Journal Articles

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