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タイトル: | Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue |
著者: | Sakaguchi, Hideya Kadoshima, Taisuke Soen, Mika Narii, Nobuhiro Ishida, Yoshihito Ohgushi, Masatoshi https://orcid.org/0000-0002-1293-4893 (unconfirmed) Takahashi, Jun Eiraku, Mototsugu Sasai, Yoshiki |
著者名の別形: | 坂口, 秀哉 高橋, 淳 |
キーワード: | Biological sciences Cell biology Developmental biology |
発行日: | 17-Nov-2015 |
出版者: | Nature Publishing Group |
誌名: | Nature Communications |
巻: | 6 |
論文番号: | 8896 |
抄録: | The developing dorsomedial telencephalon includes the medial pallium, which goes on to form the hippocampus. Generating a reliable source of human hippocampal tissue is an important step for cell-based research into hippocampus-related diseases. Here we show the generation of functional hippocampal granule- and pyramidal-like neurons from self-organizing dorsomedial telencephalic tissue using human embryonic stem cells (hESCs). First, we develop a hESC culture method that utilizes bone morphogenetic protein (BMP) and Wnt signalling to induce choroid plexus, the most dorsomedial portion of the telencephalon. Then, we find that titrating BMP and Wnt exposure allowed the self-organization of medial pallium tissues. Following long-term dissociation culture, these dorsomedial telencephalic tissues give rise to Zbtb20+/Prox1+ granule neurons and Zbtb20+/KA1+ pyramidal neurons, both of which were electrically functional with network formation. Thus, we have developed an in vitro model that recapitulates human hippocampus development, allowing the generation of functional hippocampal granule- and pyramidal-like neurons. |
著作権等: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
URI: | http://hdl.handle.net/2433/210235 |
DOI(出版社版): | 10.1038/ncomms9896 |
PubMed ID: | 26573335 |
出現コレクション: | 学術雑誌掲載論文等 |
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