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j.stemcr.2015.11.010.pdf | 3.38 MB | Adobe PDF | 見る/開く |
タイトル: | Directed Induction of Functional Multi-ciliated Cells in Proximal Airway Epithelial Spheroids from Human Pluripotent Stem Cells |
著者: | Konishi, Satoshi Gotoh, Shimpei Tateishi, Kazuhiro Yamamoto, Yuki Korogi, Yohei Nagasaki, Tadao Matsumoto, Hisako Muro, Shigeo Hirai, Toyohiro Ito, Isao https://orcid.org/0000-0003-3109-898X (unconfirmed) Tsukita, Sachiko Mishima, Michiaki |
著者名の別形: | 小西, 哲之 後藤, 慎平 月田, 早智子 松本, 久子 室, 繁郎 平井, 豊博 伊藤, 功朗 三嶋, 理晃 |
発行日: | 12-Jan-2016 |
出版者: | Elsevier Inc. |
誌名: | Stem Cell Reports |
巻: | 6 |
号: | 1 |
開始ページ: | 18 |
終了ページ: | 25 |
抄録: | Multi-ciliated airway cells (MCACs) play a role in mucociliary clearance of the lung. However, the efficient induction of functional MCACs from human pluripotent stem cells has not yet been reported. Using carboxypeptidase M (CPM) as a surface marker of NKX2-1+-ventralized anterior foregut endoderm cells (VAFECs), we report a three-dimensional differentiation protocol for generating proximal airway epithelial progenitor cell spheroids from CPM+ VAFECs. These spheroids could be induced to generate MCACs and other airway lineage cells without alveolar epithelial cells. Furthermore, the directed induction of MCACs and of pulmonary neuroendocrine lineage cells was promoted by adding DAPT, a Notch pathway inhibitor. The induced MCACs demonstrated motile cilia with a "9 + 2" microtubule arrangement and dynein arms capable of beating and generating flow for mucociliary transport. This method is expected to be useful for future studies on human airway disease modeling and regenerative medicine. |
記述: | ヒトiPS細胞から効率よく気道上皮細胞を分化誘導 --気道の再生や難病の治療薬開発に向けた大きな一歩--. 京都大学プレスリリース. 2015-12-25. |
著作権等: | © 2016 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
URI: | http://hdl.handle.net/2433/210239 |
DOI(出版社版): | 10.1016/j.stemcr.2015.11.010 |
PubMed ID: | 26724905 |
関連リンク: | http://www.kyoto-u.ac.jp/ja/research/research_results/2015/151225_1.html https://www.kyoto-u.ac.jp/ja/research-news/2015-12-25 |
出現コレクション: | 学術雑誌掲載論文等 |
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