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タイトル: | The binding specificity of Translocated in LipoSarcoma/FUsed in Sarcoma with lncRNA transcribed from the promoter region of cyclin D1 |
著者: | Yoneda, Ryoma Suzuki, Shiho Mashima, Tsukasa Kondo, Keiko https://orcid.org/0000-0003-2860-2751 (unconfirmed) Nagata, Takashi https://orcid.org/0000-0002-3733-2709 (unconfirmed) Katahira, Masato https://orcid.org/0000-0003-0336-7660 (unconfirmed) Kurokawa, Riki |
著者名の別形: | 真嶋, 司 近藤, 敬子 永田, 崇 片平, 正人 |
キーワード: | TLS/FUS Long noncoding RNA pncRNA |
発行日: | 25-Jan-2016 |
出版者: | BioMed Central Ltd. |
誌名: | Cell and Bioscience |
巻: | 6 |
論文番号: | 4 |
抄録: | Background: Translocated in LipoSarcoma (TLS, also known as FUsed in Sarcoma) is an RNA/DNA binding protein whose mutation cause amyotrophic lateral sclerosis. In previous study, we demonstrated that TLS binds to long noncoding RNA, promoter-associated ncRNA-D (pncRNA-D), transcribed from the 5' upstream region of cyclin D1 (CCND1), and inhibits the expression of CCND1. Results: In order to elucidate the binding specificity between TLS and pncRNA-D, we divided pncRNA-D into seven fragments and examined the binding with full-length TLS, TLS-RGG2-zinc finger-RGG3, and TLS-RGG3 by RNA pull down assay. As a result, TLS was able to bind to all the seven fragments, but the fragments containing reported recognition motifs (GGUG and GGU) tend to bind more solidly. The full-length TLS and TLS-RGG2-zinc finger-RGG3 showed a similar interaction with pncRNA-D, but the binding specificity of TLS-RGG3 was lower compared to the full-length TLS and TLS-RGG2-zinc finger-RGG3. Mutation in GGUG and GGU motifs dramatically decreased the binding, and unexpectedly, we could only detect weak interaction with the RNA sequence with stem loop structure. Conclusion: The binding of TLS and pncRNA-D was affected by the presence of GGUG and GGU sequences, and the C terminal domains of TLS function in the interaction with pncRNA-D. |
著作権等: | © 2016 Yoneda et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
URI: | http://hdl.handle.net/2433/214478 |
DOI(出版社版): | 10.1186/s13578-016-0068-8 |
PubMed ID: | 26816614 |
出現コレクション: | 学術雑誌掲載論文等 |
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