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タイトル: The binding specificity of Translocated in LipoSarcoma/FUsed in Sarcoma with lncRNA transcribed from the promoter region of cyclin D1
著者: Yoneda, Ryoma
Suzuki, Shiho
Mashima, Tsukasa  kyouindb  KAKEN_id
Kondo, Keiko
Nagata, Takashi  kyouindb  KAKEN_id
Katahira, Masato  kyouindb  KAKEN_id
Kurokawa, Riki
著者名の別形: 真嶋, 司
近藤, 敬子
永田, 崇
片平, 正人
キーワード: TLS/FUS
Long noncoding RNA
pncRNA
発行日: 25-Jan-2016
出版者: BioMed Central Ltd.
誌名: Cell and Bioscience
巻: 6
論文番号: 4
抄録: Background: Translocated in LipoSarcoma (TLS, also known as FUsed in Sarcoma) is an RNA/DNA binding protein whose mutation cause amyotrophic lateral sclerosis. In previous study, we demonstrated that TLS binds to long noncoding RNA, promoter-associated ncRNA-D (pncRNA-D), transcribed from the 5' upstream region of cyclin D1 (CCND1), and inhibits the expression of CCND1. Results: In order to elucidate the binding specificity between TLS and pncRNA-D, we divided pncRNA-D into seven fragments and examined the binding with full-length TLS, TLS-RGG2-zinc finger-RGG3, and TLS-RGG3 by RNA pull down assay. As a result, TLS was able to bind to all the seven fragments, but the fragments containing reported recognition motifs (GGUG and GGU) tend to bind more solidly. The full-length TLS and TLS-RGG2-zinc finger-RGG3 showed a similar interaction with pncRNA-D, but the binding specificity of TLS-RGG3 was lower compared to the full-length TLS and TLS-RGG2-zinc finger-RGG3. Mutation in GGUG and GGU motifs dramatically decreased the binding, and unexpectedly, we could only detect weak interaction with the RNA sequence with stem loop structure. Conclusion: The binding of TLS and pncRNA-D was affected by the presence of GGUG and GGU sequences, and the C terminal domains of TLS function in the interaction with pncRNA-D.
著作権等: © 2016 Yoneda et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
URI: http://hdl.handle.net/2433/214478
DOI(出版社版): 10.1186/s13578-016-0068-8
出現コレクション:学術雑誌掲載論文等

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