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Title: The binding specificity of Translocated in LipoSarcoma/FUsed in Sarcoma with lncRNA transcribed from the promoter region of cyclin D1
Authors: Yoneda, Ryoma
Suzuki, Shiho
Mashima, Tsukasa  kyouindb  KAKEN_id
Kondo, Keiko  kyouindb  KAKEN_id  orcid (unconfirmed)
Nagata, Takashi  kyouindb  KAKEN_id  orcid (unconfirmed)
Katahira, Masato  kyouindb  KAKEN_id  orcid (unconfirmed)
Kurokawa, Riki
Author's alias: 真嶋, 司
近藤, 敬子
永田, 崇
片平, 正人
Keywords: TLS/FUS
Long noncoding RNA
Issue Date: 25-Jan-2016
Publisher: BioMed Central Ltd.
Journal title: Cell and Bioscience
Volume: 6
Thesis number: 4
Abstract: Background: Translocated in LipoSarcoma (TLS, also known as FUsed in Sarcoma) is an RNA/DNA binding protein whose mutation cause amyotrophic lateral sclerosis. In previous study, we demonstrated that TLS binds to long noncoding RNA, promoter-associated ncRNA-D (pncRNA-D), transcribed from the 5' upstream region of cyclin D1 (CCND1), and inhibits the expression of CCND1. Results: In order to elucidate the binding specificity between TLS and pncRNA-D, we divided pncRNA-D into seven fragments and examined the binding with full-length TLS, TLS-RGG2-zinc finger-RGG3, and TLS-RGG3 by RNA pull down assay. As a result, TLS was able to bind to all the seven fragments, but the fragments containing reported recognition motifs (GGUG and GGU) tend to bind more solidly. The full-length TLS and TLS-RGG2-zinc finger-RGG3 showed a similar interaction with pncRNA-D, but the binding specificity of TLS-RGG3 was lower compared to the full-length TLS and TLS-RGG2-zinc finger-RGG3. Mutation in GGUG and GGU motifs dramatically decreased the binding, and unexpectedly, we could only detect weak interaction with the RNA sequence with stem loop structure. Conclusion: The binding of TLS and pncRNA-D was affected by the presence of GGUG and GGU sequences, and the C terminal domains of TLS function in the interaction with pncRNA-D.
Rights: © 2016 Yoneda et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
DOI(Published Version): 10.1186/s13578-016-0068-8
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