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Title: Basic biological characterization of feline morbillivirus
Authors: Koide, Rie
Sakaguchi, Shoichi
Miyazawa, Takayuki  kyouindb  KAKEN_id
Author's alias: 宮沢, 孝幸
Keywords: Feline
Feline morbillivirus
Quantification
Replication kinetics
Stability
Issue Date: 26-Jan-2015
Publisher: Japanese Society of Veterinary Science
Journal title: Journal of Veterinary Medical Science
Volume: 77
Issue: 5
Start page: 565
End page: 569
Abstract: Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene<sup>®</sup> or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.
Rights: ©2015 The Japanese Society of Veterinary Science. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/3.0/>.
URI: http://hdl.handle.net/2433/215064
DOI(Published Version): 10.1292/jvms.14-0623
PubMed ID: 25649267
Appears in Collections:Journal Articles

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