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Title: Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs.
Authors: Yamashita, Akihiro  kyouindb  KAKEN_id
Morioka, Miho
Yahara, Yasuhito
Okada, Minoru
Kobayashi, Tomohito
Kuriyama, Shinichi  kyouindb  KAKEN_id
Matsuda, Shuichi  kyouindb  KAKEN_id
Tsumaki, Noriyuki
Author's alias: 松田, 秀一
Issue Date: Mar-2015
Publisher: Elsevier B.V.
Journal title: Stem Cell Reports
Volume: 4
Issue: 3
Start page: 404
End page: 418
Abstract: Defects in articular cartilage ultimately result in loss of joint function. Repairing cartilage defects requires cell sources. We developed an approach to generate scaffoldless hyaline cartilage from human induced pluripotent stem cells (hiPSCs). We initially generated an hiPSC line that specifically expressed GFP in cartilage when teratoma was formed. We optimized the culture conditions and found BMP2, transforming growth factor β1 (TGF-β1), and GDF5 critical for GFP expression and thus chondrogenic differentiation of the hiPSCs. The subsequent use of scaffoldless suspension culture contributed to purification, producing homogenous cartilaginous particles. Subcutaneous transplantation of the hiPSC-derived particles generated hyaline cartilage that expressed type II collagen, but not type I collagen, in immunodeficiency mice. Transplantation of the particles into joint surface defects in immunodeficiency rats and immunosuppressed mini-pigs indicated that neocartilage survived and had potential for integration into native cartilage. The immunodeficiency mice and rats suffered from neither tumors nor ectopic tissue formation. The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects.
Rights: ©2015 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/215146
DOI(Published Version): 10.1016/j.stemcr.2015.01.016
PubMed ID: 25733017
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