Downloads: 87

Files in This Item:
File Description SizeFormat 
ncomms11858.pdf2.34 MBAdobe PDFView/Open
Title: Interphase adhesion geometry is transmitted to an internal regulator for spindle orientation via caveolin-1
Authors: Matsumura, Shigeru
Kojidani, Tomoko
Kamioka, Yuji
Uchida, Seiichi
Haraguchi, Tokuko
Kimura, Akatsuki
Toyoshima, Fumiko  kyouindb  KAKEN_id
Author's alias: 松村, 繁
豊島, 文子
Keywords: Biological sciences
Cell biology
Issue Date: 13-Jun-2016
Publisher: Nature Publishing Group
Journal title: Nature Communications
Volume: 7
Thesis number: 11858
Abstract: Despite theoretical and physical studies implying that cell-extracellular matrix adhesion geometry governs the orientation of the cell division axis, the molecular mechanisms that translate interphase adhesion geometry to the mitotic spindle orientation remain elusive. Here, we show that the cellular edge retraction during mitotic cell rounding correlates with the spindle axis. At the onset of mitotic cell rounding, caveolin-1 is targeted to the retracting cortical region at the proximal end of retraction fibres, where ganglioside GM1-enriched membrane domains with clusters of caveola-like structures are formed in an integrin and RhoA-dependent manner. Furthermore, Gαi1-LGN-NuMA, a well-known regulatory complex of spindle orientation, is targeted to the caveolin-1-enriched cortical region to guide the spindle axis towards the cellular edge retraction. We propose that retraction-induced cortical heterogeneity of caveolin-1 during mitotic cell rounding sets the spindle orientation in the context of adhesion geometry.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
DOI(Published Version): 10.1038/ncomms11858
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks

Export Format: 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.