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Title: Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates
Authors: Kawamura, Takuji
Miyagawa, Shigeru
Fukushima, Satsuki
Maeda, Akira
Kashiyama, Noriyuki
Kawamura, Ai
Miki, Kenji  kyouindb  KAKEN_id
Okita, Keisuke
Yoshida, Yoshinori  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5511-9090 (unconfirmed)
Shiina, Takashi
Ogasawara, Kazumasa
Miyagawa, Shuji
Toda, Koichi
Okuyama, Hiroomi
Sawa, Yoshiki
Author's alias: 沖田, 圭介
Issue Date: 8-Mar-2016
Publisher: Elsevier BV
Journal title: Stem cell reports
Volume: 6
Issue: 3
Start page: 312
End page: 320
Abstract: Induced pluripotent stem cells (iPSCs) can serve as a source of cardiomyocytes (CMs) to treat end-stage heart failure; however, transplantation of genetically dissimilar iPSCs even within species (allogeneic) can induce immune rejection. We hypothesized that this might be limited by matching the major histocompatibility complex (MHC) antigens between the donor and the recipient. We therefore transplanted fluorescence-labeled (GFP) iPSC-CMs donated from a macaque with homozygous MHC haplotypes into the subcutaneous tissue and hearts of macaques having heterozygous MHC haplotypes (MHC-matched; group I) or without identical MHC alleles (group II) in conjunction with immune suppression. Group I displayed a higher GFP intensity and less immune-cell infiltration in the graft than group II. However, MHC-matched transplantation with single or no immune-suppressive drugs still induced a substantial host immune response to the graft. Thus, the immunogenicity of allogeneic iPSC-CMs was reduced by MHC-matched transplantation although a requirement for appropriate immune suppression was retained for successful engraftment.
Rights: © 2016 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/216335
DOI(Published Version): 10.1016/j.stemcr.2016.01.012
PubMed ID: 26905198
Appears in Collections:Journal Articles

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