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タイトル: | The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression |
著者: | Fukumura, Kazuhiro Wakabayashi, Shunichi Kataoka, Naoyuki Sakamoto, Hiroshi Suzuki, Yutaka Nakai, Kenta Mayeda, Akila Inoue, Kunio |
著者名の別形: | 片岡, 直行 |
キーワード: | exon junction complex (EJC) Y14 pre-mRNA splicing mitotic cell-cycle |
発行日: | 2-Aug-2016 |
出版者: | MDPI AG |
誌名: | International Journal of Molecular Sciences |
巻: | 17 |
号: | 8 |
論文番号: | 1153 |
抄録: | The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes. |
著作権等: | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License (CC BY 4.0). |
URI: | http://hdl.handle.net/2433/216506 |
DOI(出版社版): | 10.3390/ijms17081153 |
PubMed ID: | 27490541 |
出現コレクション: | 学術雑誌掲載論文等 |
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