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タイトル: The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression
著者: Fukumura, Kazuhiro
Wakabayashi, Shunichi
Kataoka, Naoyuki
Sakamoto, Hiroshi
Suzuki, Yutaka
Nakai, Kenta
Mayeda, Akila
Inoue, Kunio
著者名の別形: 片岡, 直行
キーワード: exon junction complex (EJC)
Y14
pre-mRNA splicing
mitotic cell-cycle
発行日: 2-Aug-2016
出版者: MDPI AG
誌名: International Journal of Molecular Sciences
巻: 17
号: 8
論文番号: 1153
抄録: The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
著作権等: © 2016 by the authors; licensee MDPI, Basel, Switzerland.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 4.0).
URI: http://hdl.handle.net/2433/216506
DOI(出版社版): 10.3390/ijms17081153
PubMed ID: 27490541
出現コレクション:学術雑誌掲載論文等

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