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Title: 13C/15N-Enriched l -Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice
Authors: Yamada, Hisatsugu
Kameda, Tetsuro
Kimura, Yu  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5318-9255 (unconfirmed)
Imai, Hirohiko  kyouindb  KAKEN_id
Matsuda, Tetsuya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-2339-1521 (unconfirmed)
Sando, Shinsuke
Toshimitsu, Akio
Aoyama, Yasuhiro
Kondo, Teruyuki
Author's alias: 今井, 宏彦
松田, 哲也
Keywords: dopamine
L-dopa
metabolic analysis
neurotransmitters
stable isotope enrichment
triple-resonance NMR
Issue Date: 1-Apr-2016
Publisher: Wiley-VCH Verlag
Journal title: ChemistryOpen
Volume: 5
Issue: 2
Start page: 125
End page: 128
Abstract: In an attempt to monitor μm-level trace constituents, we applied here 1H-{13C-15N} triple-resonance nuclear magnetic resonance (NMR) to 13C/15N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of -30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. This work suggests that μm-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atom(s) and their appropriate 13C/15N-enrichment is synthetically accessible. Traces via triple resonance! Tripleresonance high-resolution NMR with a cryogenic probe at 16.4 T is sensitive and selective enough to perform quantitative metabolic/pharmacokinetic analysis of l-Dopa/dopamine in brain and liver extracts from mice. This work suggests that μm-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atoms and their appropriate 13C/15N-enrichment is synthetically accessible.
Rights: © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
URI: http://hdl.handle.net/2433/216667
DOI(Published Version): 10.1002/open.201500196
PubMed ID: 27308224
Appears in Collections:Journal Articles

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