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Title: Heat stress acutely activates insulin-independent glucose transport and 5′-AMP-activated protein kinase prior to an increase in HSP72 protein in rat skeletal muscle
Authors: Goto, Ayumi
Egawa, Tatsuro  kyouindb  KAKEN_id
Sakon, Ichika
Oshima, Rieko
Ito, Kanata
Serizawa, Yasuhiro
Sekine, Keiichi
Tsuda, Satoshi
Goto, Katsumasa
Hayashi, Tatsuya  kyouindb  KAKEN_id
Author's alias: 林, 達也
Keywords: Epitrochlearis
glucose metabolism
GLUT4
hyperthermia
Issue Date: 5-Nov-2015
Publisher: Wiley-Blackwell Publishing Ltd
Journal title: Physiological Reports
Volume: 3
Issue: 11
Thesis number: e12601
Abstract: Heat stress (HS) stimulates heat shock protein (HSP) 72 mRNA expression, and the period after an increase in HSP72 protein is characterized by enhanced glucose metabolism in skeletal muscle. We have hypothesized that, prior to an increase in the level of HSP72 protein, HS activates glucose metabolism by acutely stimulating 5′-AMP-activated protein kinase (AMPK). Rat epitrochlearis muscle was isolated and incubated either with or without HS (42°C) for 10 and 30 min. HS for 30 min led to an increase in the level of Hspa1a and Hspa1b mRNA but did not change the amount of HSP72 protein. However, HS for both 10 and 30 min led to a significant increase in the rate of 3-O-methyl-d-glucose (3MG) transport, and the stimulatory effect of 3MG transport was completely blocked by cytochalasin B. HS-stimulated 3MG transport was also inhibited by dorsomorphin but not by wortmannin. HS led to a decrease in the concentration of ATP, phosphocreatine, and glycogen, to an increase in the level of phosphorylation of AMPKα Thr[172], and to an increase in the activity of both AMPKα1 and AMPKα2. HS did not affect the phosphorylation status of insulin receptor signaling or Ca[2+]/calmodulin-dependent protein kinase II. These results suggest that HS acts as a rapid stimulator of insulin-independent glucose transport, at least in part by stimulating AMPK via decreased energy status. Although further research is warranted, heat treatment of skeletal muscle might be a promising method to promote glucose metabolism acutely.
Rights: © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/216670
DOI(Published Version): 10.14814/phy2.12601
PubMed ID: 26542263
Appears in Collections:Journal Articles

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