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  2. 110_Graduate School of Human and Environmental Studies
  3. Professor Emeritus
  4. 松井 正文
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    Please use this identifier to cite or link to this item: http://hdl.handle.net/2433/216862
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dc.contributor.authorEto, Koshiroen
dc.contributor.authorMatsui, Masafumien
dc.contributor.authorNishikawa, Kantoen
dc.contributor.authorHaramura, Takashien
dc.contributor.alternative松井, 正文ja
dc.date.accessioned2016-10-05T02:27:16Z-
dc.date.available2016-10-05T02:27:16Z-
dc.date.issued2016-02-
dc.identifier.issn1345-5834-
dc.identifier.urihttp://hdl.handle.net/2433/216862-
dc.description.abstractThree species/subspecies of toads (Bufo japonicus japonicus, B. j. formosus, and B. torrenticola) show parapatric/sympatric patterns of distribution in Japan, and usually they are morphologically indistinguishable at early developmental stages. For quick and easy genetic identification of these three toads, we validated LAMP assay. We designed taxon-specific primers for LAMP assay on mitochondrial cytochrome b region, and these primer sets accurately assigned DNA samples derived from three toads to each taxon. Due to its instancy and simplicity, LAMP method could be a useful diagnostic tool to distinguish taxa which resemble each other.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherHerpetological Society of Japanen
dc.publisher.alternative日本爬虫両棲類学会ja
dc.rights© 2016 by The Herpetological Society of Japanen
dc.rightsThe full-text file will be made open to the public on 2018-02-01 in accordance with publisher's 'Terms and Conditions for Self-Archiving'en
dc.subjectLAMPen
dc.subjectBufoen
dc.subjectCytochrome ben
dc.subjectSpecies identificationen
dc.titleDevelopment and Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for Quick Identification of Three Japanese Toadsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.ncidAA12410796-
dc.identifier.jtitleCurrent Herpetologyen
dc.identifier.volume35-
dc.identifier.issue1-
dc.identifier.spage33-
dc.identifier.epage37-
dc.relation.doi10.5358/hsj.35.33-
dc.textversionpublisher-
dc.startdate.bitstreamsavailable2018-02-01-
dcterms.accessRightsopen access-
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